Najeeb Hishyar Azo, Othman Ramadhan, Salih Sherwan F, Mohammed Ayad Ahmad, Ismaeel Qais Al
Department of Medical Chemistry, College of Medicine, University of Duhok, Kurdistan Region, Iraq.
Department of Internal Medicine, College of Medicine, University of Duhok, Kurdistan Region, Iraq.
Ann Med Surg (Lond). 2020 Nov 10;60:462-467. doi: 10.1016/j.amsu.2020.10.065. eCollection 2020 Dec.
Many clinical and pre-clinical studies suggested the protective effect of vitamin D against cancer development and cancer progression. Vitamin D deficiency is highly prevalent worldwide, and its link to DNA damage is worthy to study. It has been shown that vitamin D supplementation can reduce the risk of cancer with a favorable prognosis. Studies on DNA damage in different types of cancer and its link to plasma vitamin D has not been found in literature.
In this study we included 45 patients with different types of cancers and 35 healthy individuals as controls. The plasma vitamin D levels were measured in all participants. DNA damage levels of peripheral blood (mononuclear) cells in 45 newly diagnosed and untreated cancer patients and in 35 healthy individuals were measured using Alkaline Comet Assay technique.
The DNA damage observed in cancer patients was significantly higher than in healthy individuals. Interestingly, we have found a significant inverse correlation between the plasma levels of vitamin D and DNA damage in cancer patients (p < 0.0001) and in healthy individuals (p < 0.001).
There is an inverse association between endogenous DNA damage and plasma vitamin D levels. Patients with vitamin D deficiency show highest levels of DNA damage suggesting that deficiency of vitamin D is probably one of the factors which increases the risk of cancer.
许多临床和临床前研究表明维生素D对癌症发生和癌症进展具有保护作用。维生素D缺乏在全球范围内高度普遍，其与DNA损伤的关联值得研究。已表明补充维生素D可降低癌症风险并带来良好预后。尚未在文献中发现关于不同类型癌症中DNA损伤及其与血浆维生素D关联的研究。
在本研究中，我们纳入了45例不同类型癌症患者和35名健康个体作为对照。测量了所有参与者的血浆维生素D水平。采用碱性彗星试验技术测量了45例新诊断且未接受治疗的癌症患者及35名健康个体外周血（单核）细胞的DNA损伤水平。
癌症患者中观察到的DNA损伤显著高于健康个体。有趣的是，我们发现癌症患者（p < 0.0001）和健康个体（p < 0.001）的血浆维生素D水平与DNA损伤之间存在显著负相关。
内源性DNA损伤与血浆维生素D水平之间存在负相关。维生素D缺乏的患者表现出最高水平的DNA损伤，这表明维生素D缺乏可能是增加癌症风险的因素之一。
