Khansaritoreh Elmira, Salmaki Yasaman, Ramezani Elias, Akbari Azirani Tayebeh, Keller Alexander, Neumann Katrin, Alizadeh Kamaleddin, Zarre Shahin, Beckh Gudrun, Behling Hermann
University of Goettingen, Department of Palynology and Climate Dynamics, Untere Karspüle 2, 37073, Goettingen, Germany.
Department of Plant Science, Center of Excellence in Phylogeny, School of Biology, College of Science, University of Tehran, P.O. Box 14155-6455, Tehran, Iran.
Heliyon. 2020 Nov 25;6(11):e05596. doi: 10.1016/j.heliyon.2020.e05596. eCollection 2020 Nov.
Unfavourable climatic conditions force Iranian beekeepers to translocate over large distances in the course of the year. However, irrespective of the main place of production, the honey is always labeled with the name of the beekeepers' hometown, which leads consequently to mislabeled products. The present study investigates the capability of DNA metabarcoding to locate the geographical origin of honey. The molecular markers (ITS2 and ) allowed identification of 926 plant species in studied samples. A comprehensive review of floristic reference books specified 34 key species that could be used to successfully determine the geographical origin in 91.4% of samples. These key species were usually present in honey with tiny amounts and thus, conventional palynology might not be able to detect them. The present investigation indicates that although ITS2 is able to detect more species than , utilizing a combination of both markers provides more robust evidence of geographical origin.
不利的气候条件迫使伊朗养蜂人在一年中长途迁移。然而，无论主要产地在哪里，蜂蜜总是标注养蜂人的家乡名称，结果导致产品标签错误。本研究调查了DNA宏条形码技术定位蜂蜜地理来源的能力。分子标记（ITS2和……）使我们能够在所研究的样本中鉴定出926种植物物种。对植物志参考书的全面回顾确定了34种关键物种，这些物种可用于成功确定91.4%的样本的地理来源。这些关键物种在蜂蜜中通常含量极少，因此传统孢粉学可能无法检测到它们。本研究表明，尽管ITS2比……能检测到更多物种，但同时使用这两种标记能提供更有力的地理来源证据。 （注：原文中“and ”处信息缺失）
