Sun Kun, Li Lishi, Ma Li, Zhao Yu, Deng Lin, Wang Huating, Sun Hao
Shenzhen Bay Laboratory, Shenzhen 518132, China.
Peking University Shenzhen Graduate School, Shenzhen 518055, China.
Patterns (N Y). 2020 Oct 15;1(8):100127. doi: 10.1016/j.patter.2020.100127. eCollection 2020 Nov 13.
DNA methylation is a pervasive and important epigenetic regulator in mammalian genome. For DNA methylome profiling, emerging bisulfite-free methods have demonstrated desirable superiority over the conventional bisulfite-treatment-based approaches, although current analysis software could not make full use of their advantages. In this work, we present Msuite, an easy-to-use, all-in-one data-analysis toolkit. Msuite implements a unique 4-letter analysis mode specifically optimized for emerging protocols; it also integrates quality controls, methylation call, and data visualizations. Msuite demonstrates substantial performance improvements over current state-of-the-art tools as well as fruitful functionalities, thus holding the potential to serve as an optimal toolkit to facilitate DNA methylome studies. Source codes and testing datasets for Msuite are freely available at https://github.com/hellosunking/Msuite/.
DNA甲基化是哺乳动物基因组中一种普遍且重要的表观遗传调控因子。对于DNA甲基化组分析而言，新兴的无亚硫酸氢盐方法已显示出相对于传统的基于亚硫酸氢盐处理方法的显著优势，尽管目前的分析软件无法充分利用其优势。在这项工作中，我们展示了Msuite，这是一个易于使用的一体化数据分析工具包。Msuite实现了一种专门为新兴方案优化的独特四字母分析模式；它还集成了质量控制、甲基化调用和数据可视化功能。Msuite相较于当前最先进的工具在性能上有显著提升，且功能丰富，因此有潜力成为促进DNA甲基化组研究的最佳工具包。Msuite的源代码和测试数据集可在https://github.com/hellosunking/Msuite/上免费获取。
