School of Life Science and Technology, ShanghaiTech University, 201210, Shanghai, China.
School of Life Science and Technology, ShanghaiTech University, 201210, Shanghai, China; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, OX1 3PT, United Kingdom.
Exp Cell Res. 2022 Nov 1;420(1):113337. doi: 10.1016/j.yexcr.2022.113337. Epub 2022 Sep 7.
CTP synthase (CTPS), a metabolic enzyme responsible for the de novo synthesis of CTP, can form filamentous structures termed cytoophidia, which are evolutionarily conserved from bacteria to humans. Here we used Schizosaccharomyces pombe to study the cytoophidium assembly regulation by ubiquitination. We tested the CTP synthase's capacity to be post-translationally modified by ubiquitin or be affected by the ubiquitination state of the cell and showed that ubiquitination is important for the maintenance of the CTPS filamentous structure in fission yeast. We have identified proteins which are in complex with CTPS, including specific ubiquitination regulators which significantly affect CTPS filamentation, and mapped probable ubiquitination targets on CTPS. Furthermore, we discovered that a cohort of deubiquitinating enzymes is important for the regulation of cytoophidium's filamentous morphology. Our study provides a framework for the analysis of the effects that ubiquitination and deubiquitination have on the formation of cytoophidia.
CTP 合酶（CTPS）是一种负责从头合成 CTP 的代谢酶，能够形成丝状结构，称为细胞纤维，这种结构从细菌到人类都是进化保守的。在这里，我们使用酿酒酵母来研究泛素化对细胞纤维组装的调节。我们测试了 CTP 合酶是否可以被泛素化修饰，或者是否受细胞泛素化状态的影响，并表明泛素化对于裂殖酵母中 CTPS 丝状结构的维持很重要。我们已经鉴定出与 CTPS 相互作用的蛋白质，包括对 CTPS 丝状形成有显著影响的特定泛素化调节因子，并对 CTPS 上可能的泛素化靶标进行了定位。此外，我们发现一组去泛素化酶对细胞纤维丝状形态的调节很重要。我们的研究为分析泛素化和去泛素化对细胞纤维形成的影响提供了一个框架。
