一种用于快速检测水溶液和活细胞中亚硫酸氢盐和过氧化氢的新型双功能荧光探针。
A novel dual-function fluorescent probe for the rapid detection of bisulfite and hydrogen peroxide in aqueous solution and living cells.
作者信息
Zhou Ruqiao, Niu Longxing, Hu Yuefu, Qi Qingrong, Huang Wencai, Yang Li
机构信息
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Mar 5;248:119226. doi: 10.1016/j.saa.2020.119226. Epub 2020 Nov 18.
In this work, Hcy-OB, a novel hemicyanine-based biocompatible dual-function fluorescence probe for bisulfite and HO detection is designed and synthesized. Based on a 1,4-addition reaction, Hcy-OB can be used for bisulfite detection with fast response, high sensitivity and low detection limit (120 nM). In addition, the probe is successfully applied to the detection of bisulfite in aqueous solution. Furthermore, Hcy-OB shows excellent performance for hydrogen peroxide detection with the oxidation of phenylboronic acid. Hcy-OB shows excellent selectivity to HO over other interfering substances with detection limit of HO is calculated to be 70 nM. Most importantly, due to its good cell membrane permeability and low cytotoxicity, Hcy-OB has been applied to monitor and image HO in living cells and mice.
在本工作中，设计并合成了一种新型的基于半菁的生物相容性双功能荧光探针Hcy-OB，用于亚硫酸氢盐和过氧化氢（HO）的检测。基于1,4-加成反应，Hcy-OB可用于亚硫酸氢盐检测，具有响应快、灵敏度高和检测限低（120 nM）的特点。此外，该探针已成功应用于水溶液中亚硫酸氢盐的检测。此外，Hcy-OB在通过苯硼酸氧化检测过氧化氢方面表现出优异性能。Hcy-OB对HO的选择性优于其他干扰物质，计算得出HO的检测限为70 nM。最重要的是，由于其良好的细胞膜通透性和低细胞毒性，Hcy-OB已应用于活细胞和小鼠中HO的监测与成像。
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