通过使用含有质粒复制子的mini-Mu-lac进行克隆来鉴定编码O-乙酰丝氨酸巯基酶B的大肠杆菌cysM基因。
Identification of the Escherichia coli cysM gene encoding O-acetylserine sulphydrylase B by cloning with mini-Mu-lac containing a plasmid replicon.
作者信息
Sirko A E, Zatyka M, Hulanicka M D
机构信息
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
出版信息
J Gen Microbiol. 1987 Oct;133(10):2719-25. doi: 10.1099/00221287-133-10-2719.
PMID:3329675
Abstract
A region located at around 52' on the Escherichia coli chromosome was cloned by use of mini-Mu-lac containing a plasmid replicon and recloned into pBR322. Enzyme assays on transformants carrying the cloned fragments indicated the presence in the latter of the cysA and cysM genes coding for sulphate permease and O-acetylserine sulphydrylase B, respectively.
摘要
利用含有质粒复制子的微型 Mu - lac 对大肠杆菌染色体上大约 52' 处的一个区域进行克隆，并将其亚克隆到 pBR322 中。对携带克隆片段的转化体进行酶活性测定，结果表明后者中分别存在编码硫酸盐通透酶的 cysA 基因和 O - 乙酰丝氨酸巯基酶 B 的 cysM 基因。
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