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响应引起小麦矮腥黑穗病的网腥黑粉菌的小麦穗转录组分析。

Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt.

作者信息

Ren Zhaoyu, Liu Jianjian, Din Ghulam Muhae Ud, Zhang Han, Du Zhenzhen, Chen Wanquan, Liu Taiguo, Zhang Jianmin, Zhao Sifeng, Gao Li

机构信息

State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

School of Agriculture, Yangtze University, Hubei, 434025, China.

出版信息

Sci Rep. 2020 Dec 9;10(1):21567. doi: 10.1038/s41598-020-78628-0.

DOI:10.1038/s41598-020-78628-0
PMID:33299089
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7725808/
Abstract

Wheat dwarf bunt is caused by Tilletia controversa Kühn, which is one of the most destructive diseases of wheat worldwide. To explore the interaction of T. controversa and wheat, we analysed the transcriptome profile of spikes of the susceptible wheat cultivar Dongxuan 3, which was subjected to a T. controversa infection and a mock infection. The results obtained from a differential expression analysis of T. controversa-infected plants compared with mock-infected ones showed that 10,867 out of 21,354 genes were upregulated, while 10,487 genes were downregulated, and these genes were enriched in 205 different pathways. Our findings demonstrated that the genes associated with defence against diseases, such as PR-related genes, WRKY transcription factors and mitogen-activated protein kinase genes, were more highly expressed in response to T. controversa infection. Additionally, a number of genes related to physiological attributes were expressed during infection. Three pathways were differentiated based on the characteristics of gene ontology classification. KEGG enrichment analysis showed that twenty genes were expressed differentially during the infection of wheat with T. controversa. Notable changes were observed in the transcriptomes of wheat plants after infection. The results of this study may help to elucidate the mechanism governing the interactions between this pathogen and wheat plants and may facilitate the development of new methods to increase the resistance level of wheat against T. controversa, including the overexpression of defence-related genes.

摘要

小麦矮腥黑穗病由蒂氏黑粉菌（Tilletia controversa Kühn）引起，它是全球最具破坏性的小麦病害之一。为了探究蒂氏黑粉菌与小麦的相互作用，我们分析了感病小麦品种东选3号穗部的转录组图谱，该品种分别受到蒂氏黑粉菌侵染和模拟侵染。对蒂氏黑粉菌侵染植株与模拟侵染植株进行差异表达分析的结果表明，在21354个基因中，有10867个基因上调，10487个基因下调，这些基因富集在205条不同的通路中。我们的研究结果表明，与抗病防御相关的基因，如病程相关基因、WRKY转录因子和丝裂原活化蛋白激酶基因，在应对蒂氏黑粉菌侵染时表达量更高。此外，在侵染过程中还表达了一些与生理特性相关的基因。根据基因本体分类特征区分出三条通路。KEGG富集分析表明，在小麦受蒂氏黑粉菌侵染期间有20个基因表达存在差异。侵染后小麦植株的转录组出现了显著变化。本研究结果可能有助于阐明该病原菌与小麦植株之间相互作用的调控机制，并可能有助于开发新方法来提高小麦对蒂氏黑粉菌的抗性水平，包括过表达防御相关基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e3b/7725808/f2e28ce7b212/41598_2020_78628_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e3b/7725808/7994ef84e1ce/41598_2020_78628_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e3b/7725808/f2e28ce7b212/41598_2020_78628_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e3b/7725808/7994ef84e1ce/41598_2020_78628_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e3b/7725808/da8d0ddce358/41598_2020_78628_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e3b/7725808/f0bde3c11c9c/41598_2020_78628_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e3b/7725808/17035d11fbc7/41598_2020_78628_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e3b/7725808/f08fce78e491/41598_2020_78628_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e3b/7725808/3c69b59c3d70/41598_2020_78628_Fig6_HTML.jpg
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