State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
Microbiome Research Center, Moon (Guangzhou) Biotech Ltd., Guangzhou, People's Republic of China.
Microbiol Spectr. 2023 Feb 14;11(1):e0039022. doi: 10.1128/spectrum.00390-22. Epub 2023 Jan 10.
Wheat dwarf bunt leads to the replacement of seeds with fungal galls containing millions of teliospores of the pathogen Tilletia controversa Kühn. As one of the most devastating internationally quarantined wheat diseases, wheat dwarf bunt spreads to cause distant outbreaks by seeds containing teliospores. In this study, based on a combination of amplicon sequencing and isolation approaches, we analyzed the seed microbiome signatures of endophytes between resistant and susceptible cultivars after infection with T. controversa. Among 310 bacterial species obtained only by amplicon sequencing and 51 species obtained only by isolation, we found 14 overlapping species by both methods; we detected 128 fungal species only by amplicon sequencing, 56 only by isolation, and 5 species by both methods. The results indicated that resistant uninfected cultivars hosted endophytic communities that were much more stable and beneficial to plant health than those in susceptible infected cultivars. The susceptible group showed higher diversity than the resistant group, the infected group showed more diversity than the uninfected group, and the microbial communities in seeds were related to infection or resistance to the pathogen. Some antagonistic microbes significantly suppressed the germination rate of the pathogen's teliospores, providing clues for future studies aimed at developing strategies against wheat dwarf bunt. Collectively, this research advances the understanding of the microbial assembly of wheat seeds upon exposure to fungal pathogen (T. controversa) infection. This is the first study on the microbiome signature of endophytes in wheat seed response to wheat dwarf bunt caused by Tilletia controversa Kühn. Some antagonistic microbes suppressed the germination of teliospores of the pathogen significantly, which will provide clues for future studies against wheat dwarf bunt. Collectively, this research first advances the understanding of the microbial assembly of wheat seed upon exposure to the fungal pathogen (T. controversa) infection.
小麦矮腥黑粉病导致种子被真菌瘿包含数以百万计的病原菌腥黑粉菌休眠孢子所取代。作为国际上最具破坏性的检疫性小麦病害之一，小麦矮腥黑粉病通过含有休眠孢子的种子传播，导致远距离爆发。在这项研究中，我们基于扩增子测序和分离方法的结合，分析了感染腥黑粉菌后抗性和感病品种种子内生菌的微生物组特征。在仅通过扩增子测序获得的 310 种细菌物种和仅通过分离获得的 51 种细菌物种中，我们通过两种方法发现了 14 个重叠物种；我们仅通过扩增子测序检测到 128 种真菌物种，仅通过分离获得 56 种，通过两种方法获得 5 种。结果表明，抗性未感染品种的内生群落比感病感染品种的内生群落更稳定，更有利于植物健康。易感组的多样性高于抗性组，感染组的多样性高于未感染组，种子中的微生物群落与对病原体的感染或抗性有关。一些拮抗微生物显著抑制了病原菌休眠孢子的萌发率，为未来开发防治小麦矮腥黑粉病的策略提供了线索。总的来说，这项研究推进了我们对小麦种子暴露于真菌病原体（腥黑粉菌）感染后微生物组装的理解。这是首次研究小麦内生菌对由腥黑粉菌引起的小麦矮腥黑粉病的微生物组特征。一些拮抗微生物显著抑制了病原菌休眠孢子的萌发，这将为未来防治小麦矮腥黑粉病的研究提供线索。总的来说，这项研究首次推进了我们对小麦种子暴露于真菌病原体（腥黑粉菌）感染后微生物组装的理解。
