小麦品种对 J. G. Kühn 的反应——使用 qRT-PCR 和激光共聚焦显微镜。

Wheat Varietal Response to J. G. Kühn Using qRT-PCR and Laser Confocal Microscopy.

机构信息

College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China.

State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

出版信息

Genes (Basel). 2021 Mar 16;12(3):425. doi: 10.3390/genes12030425.

J. G. Kühn is a causal organism of dwarf bunt in wheat. Understanding the interaction of wheat and is of practical and scientific importance for disease control. In this study, the relative expression of and and genes was higher in a resistant (Yinong 18) and moderately resistant (Pin 9928) cultivars rather than susceptible (Dongxuan 3) cultivar at 72 h post inoculation (hpi) with . Similarly, the expression of defensin, and genes was observed higher in resistant and moderately resistant cultivars after exogenous application of phytohormones, including methyl jasmonate, salicylic acid, and abscisic acid. Laser confocal microscopy was used to track the fungal hyphae in the roots, leaves, and tapetum cells, which of susceptible cultivar were infected harshly by than moderately resistant and resistant cultivars. There were no fungal hyphae in tapetum cells in susceptible cultivar after methyl jasmonate, salicylic acid and abscisic acid treatments. Moreover, after infection, the pollen germination was of 80.06, 58.73, and 0.67% in resistant, moderately resistant and susceptible cultivars, respectively. The above results suggested that the use using of resistant cultivar is a good option against the dwarf bunt disease.

J.G. Kühn 是小麦矮腥黑穗病菌的致病因子。了解小麦与之间的相互作用对于疾病控制具有实际和科学意义。在这项研究中，与感病品种（东选 3）相比，在接种 72 小时后，抗性（引农 18）和中度抗性（品 9928）品种中和基因的相对表达水平更高。同样，在经过外源施用茉莉酸甲酯、水杨酸和脱落酸等植物激素处理后，抗性和中度抗性品种中防御素、和基因的表达也观察到更高。激光共聚焦显微镜用于跟踪根部、叶片和绒毡层细胞中的真菌菌丝，感病品种中的真菌菌丝比中度抗性和抗性品种更容易感染。在感病品种中，茉莉酸甲酯、水杨酸和脱落酸处理后，绒毡层细胞中没有真菌菌丝。此外，在感染后，抗性、中度抗性和感病品种的花粉萌发率分别为 80.06%、58.73%和 0.67%。上述结果表明，使用抗性品种是防治小麦矮腥黑穗病的一种较好选择。