Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid 22110, Jordan.
Institute of Anatomy II, Medical Faculty, Heinrich Heine Universität, Düsseldorf, Germany.
Drug Des Devel Ther. 2020 Dec 1;14:5299-5314. doi: 10.2147/DDDT.S278153. eCollection 2020.
Chronic psychosocial stress impairs memory function and leads to a depression-like phenotype induced by a persistent status of oxidative stress. L. (St. John's wort) is widely used to relieve symptoms of anxiety and depression; however, its long-term use is associated with adverse effects. Turra is closely related to . Both plants belong to family and share many biologically active compounds. Previous work by our group showed that methanolic extracts of have potent antioxidant activity as well as high hypericin content, a component that proved to have stress-relieving and antidepressant effects by other studies. Therefore, we hypothesized that would reduce stress-induced cognitive impairment in a rat model of chronic stress.
To determine whether chronic treatment with protects against stress-associated memory deficits and to investigate a possible mechanism.
The radial arm water maze (RAWM) was used to test learning and memory in rats exposed to daily stress using the resident-intruder paradigm. Stressed and unstressed rats received chronic or vehicle. We also measured levels of brain-derived neurotrophic factor (BDNF) in the hippocampus, cortex and cerebellum.
Neither chronic stress nor chronic administration affected performance during acquisition. However, memory tests in the RAWM showed that chronic stress impaired different post-encoding memory stages. prevented this impairment. Furthermore, hippocampal BDNF levels were markedly lower in stressed animals than in unstressed animals, and chronic administration of chronic administration protected against this reduction. No significant difference was observed in the effects of chronic stress and/or treatment on BDNF levels in the cerebellum and cortex.
extract can oppose stress-associated hippocampus-dependent memory deficits in a mechanism that may involve BDNF in the hippocampus.
慢性心理社会应激会损害记忆功能，并导致持续氧化应激状态引起的抑郁样表型。贯叶金丝桃（圣约翰草）被广泛用于缓解焦虑和抑郁症状；然而，长期使用与不良反应有关。Turra 与贯叶金丝桃密切相关。这两种植物都属于金丝桃科，含有许多具有生物活性的化合物。我们小组的先前工作表明，贯叶金丝桃的甲醇提取物具有很强的抗氧化活性和较高的金丝桃素含量，其他研究表明，金丝桃素具有缓解压力和抗抑郁作用。因此，我们假设贯叶金丝桃会减轻慢性应激大鼠模型中的应激引起的认知障碍。
确定慢性贯叶金丝桃治疗是否可以预防与应激相关的记忆缺陷，并探讨可能的机制。
使用放射臂水迷宫（RAWM）测试暴露于每日应激的大鼠的学习和记忆，使用居民入侵者范式。应激和非应激大鼠接受慢性贯叶金丝桃或载体治疗。我们还测量了海马体、皮质和小脑中的脑源性神经营养因子（BDNF）水平。
慢性应激或贯叶金丝桃给药均不影响获得期间的表现。然而，在 RAWM 中的记忆测试表明，慢性应激损害了不同的后编码记忆阶段。贯叶金丝桃预防了这种损伤。此外，应激动物的海马体 BDNF 水平明显低于非应激动物，而慢性贯叶金丝桃给药可防止这种降低。慢性应激和/或贯叶金丝桃治疗对小脑和皮质中 BDNF 水平的影响没有显著差异。
贯叶金丝桃提取物可以在一种可能涉及海马体 BDNF 的机制中对抗与应激相关的海马体依赖性记忆缺陷。
