Gresik E, Barka T
J Histochem Cytochem. 1977 Sep;25(9):1027-35. doi: 10.1177/25.9.333017.
The cellular and subcellular localization of epidermal growth factor in the submandibular glands of male and female adult mice was established by immunoperoxidase techniques. In light microscopic preparations epidermal growth factor was found exclusively in the granular convoluted tubules of the gland. The intensity of staining for epidermal growth factor varied from cell to cell, and some cells apparently were negative. The pattern of staining was similar in the glands of male and female mice; however, the granular convoluted tubules are androgen-responsive, and thus more extensive and composed of larger cells in males. In thin sections epidermal growth factor was most heavily concentrated in the secretion granules of the granular convoluted tubule cells. Within a given cell there was variation in intensity of staining of individual secretion granules, with some granules appearing minimally reactive or negative. The only other cell component with deposits of reaction product was the ribosomes.
采用免疫过氧化物酶技术确定了成年雄性和雌性小鼠下颌下腺中表皮生长因子的细胞和亚细胞定位。在光学显微镜标本中，表皮生长因子仅在腺体的颗粒曲管中发现。表皮生长因子的染色强度因细胞而异，一些细胞显然呈阴性。雄性和雌性小鼠腺体中的染色模式相似；然而，颗粒曲管对雄激素有反应，因此在雄性中更广泛且由更大的细胞组成。在超薄切片中，表皮生长因子最密集地集中在颗粒曲管细胞的分泌颗粒中。在给定的细胞内，单个分泌颗粒的染色强度存在差异，一些颗粒显示反应性最小或呈阴性。唯一有反应产物沉积的其他细胞成分是核糖体。
