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从变质鸡蛋中分离得到的 Dermacoccus abyssi 菌株 HZAU 226 的全基因组测序和基因组注释。

Whole genome sequencing and genome annotation of Dermacoccus abyssi strain HZAU 226 isolated from spoiled eggs.

机构信息

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430000, China.

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430000, China; National Research and Development Center for Egg Processing, Wuhan 430000, China.

出版信息

Genomics. 2021 Jan;113(1 Pt 2):1199-1206. doi: 10.1016/j.ygeno.2020.12.005. Epub 2020 Dec 8.

Abstract

Dermacoccus abyssi strain HZAU 226 is a spoilage bacterium isolated from eggs. So far, there are still few genomic resources available on the Dermacoccus abyssi. Here, we reported the complete genome sequence of Dermacoccus abyssi strain HZAU 226. High-quality DNA was extracted using the Qiagen kit, then single-molecule sequencing was performed by GridION sequencer. The raw data was quality-controlled and assembled to obtain the final genome, which consisted of a complete genome of 2,992,060 bp circular chromosome and a 64,524 bp plasmid. The structural and functional annotations of the genome were achieved through the analysis of different available databases, including antibiotic resistance genes, secondary metabolite synthesis genes and stress-related genes. Meanwhile, comparative genomic analyses of the strains were also performed. This is the first report on the complete genome of Dermacoccus abyssi, which will provide genomic resources for the study of spoilage bacteria in eggs.

摘要

深渊海球菌 HZAU 226 菌株是从鸡蛋中分离出来的一种腐败菌。迄今为止,关于深渊海球菌的基因组资源仍然很少。在这里,我们报道了深渊海球菌 HZAU 226 菌株的完整基因组序列。使用 Qiagen 试剂盒提取高质量 DNA,然后使用 GridION 测序仪进行单分子测序。对原始数据进行质量控制和组装,获得最终的基因组,该基因组由一个 2992060 bp 的圆形染色体和一个 64524 bp 的质粒组成。通过对不同可用数据库的分析,包括抗生素抗性基因、次生代谢物合成基因和应激相关基因,实现了基因组的结构和功能注释。同时,还对菌株进行了比较基因组分析。这是深渊海球菌完整基因组的首次报道,为研究鸡蛋中腐败菌提供了基因组资源。

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