Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt.
Mar Drugs. 2020 Feb 25;18(3):131. doi: 10.3390/md18030131.
strain MT1.1 is a piezotolerant actinobacterium that was isolated from Mariana Trench sediment collected at a depth of 10898 m. The organism was found to produce ten dermacozines (A‒J) that belonged to a new phenazine family and which displayed various biological activities such as radical scavenging and cytotoxicity. Here, we report on the isolation and identification of a new dermacozine compound, dermacozine M, the chemical structure of which was determined using 1D and 2D-NMR, and high resolution MS. A whole genome sequence of the strain contained six secondary metabolite-biosynthetic gene clusters (BGCs), including one responsible for the biosynthesis of a family of phenazine compounds. A pathway leading to the biosynthesis of dermacozines is proposed. Bioinformatic analyses of key stress-related genes provide an insight into how the organism adapted to the environmental conditions that prevail in the deep-sea.
菌株 MT1.1 是一种耐压的放线菌，从马里亚纳海沟沉积物中分离得到，采集深度为 10898 米。该生物被发现产生了十种真皮素（A-J），属于一种新的吩嗪家族，具有各种生物活性，如清除自由基和细胞毒性。在这里，我们报告了一种新的真皮素化合物的分离和鉴定，即真皮素 M，其化学结构通过 1D 和 2D-NMR 以及高分辨率 MS 确定。该菌株的全基因组序列包含六个次级代谢产物生物合成基因簇（BGCs），其中一个负责吩嗪化合物家族的生物合成。提出了一条真皮素生物合成途径。关键应激相关基因的生物信息学分析为我们了解该生物如何适应深海环境条件提供了线索。
