Enzyme and Microbial Biochemistry Laboratory, Department of Chemistry, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India.
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India.
Mar Biotechnol (NY). 2022 Dec;24(6):1168-1175. doi: 10.1007/s10126-022-10168-2. Epub 2022 Oct 11.
With the overuse and misuse of antibiotics amid COVID-19 pandemic, the antimicrobial resistance, which is already a global challenge, has accelerated its pace significantly. Finding novel and potential antibiotics seems one of the probable solutions. In this work, a novel Streptomyces sp. strain EMB24 was isolated and found to be an excellent source of antimicrobials as confirmed by agar-plug assay. It showed antibacterial activity against infection-causing bacteria, namely Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. In addition, Streptomyces sp. strain EMB24 inhibited the growth methicillin-resistant Staphylococcus aureus (MRSA), tetracycline-resistant Neisseria gonorrhoeae, and ampicillin-resistant Neisseria gonorrhoeae. Furthermore, to get deep insights about the genome and biosynthetic gene clusters producing antibiotics, whole genome sequencing was done. The strain EMB24 is closely related to the Streptomyces longispororuber as revealed by phylogenetic analysis which is a potential source of antibiotics and pigments as undecylprodigiosin and metacycloprodigiosin belonging to the class prodigiosin. Naphthyridinomycin, alkylresorcinols, desferrioxamine B and E, venezuelin, aborycin, MS-271, and siamycin are potent therapeutics that shared 100% similarity with the reference strain as revealed by the online antiSMASH tool.
在 COVID-19 大流行期间，由于抗生素的过度和滥用，已经是全球性挑战的抗微生物药物耐药性明显加快了步伐。寻找新型和潜在的抗生素似乎是一种可行的解决方案。在这项工作中，分离到一种新型链霉菌 EMB24 菌株，通过琼脂塞法证实它是一种极好的抗菌药物来源。它对引起感染的细菌（金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌）表现出抗菌活性。此外，链霉菌 EMB24 菌株抑制耐甲氧西林金黄色葡萄球菌 (MRSA)、耐四环素淋病奈瑟菌和耐氨苄西林淋病奈瑟菌的生长。此外，为了更深入地了解产生抗生素的基因组和生物合成基因簇，进行了全基因组测序。该菌株 EMB24 通过系统发育分析与长孢链霉菌密切相关，长孢链霉菌是抗生素和色素（如属于灵菌红素类的十一烷吡咯红和中环灵菌红素）的潜在来源。萘啶霉素、烷基间苯二酚、去铁胺 B 和 E、委内瑞拉霉素、阿博霉素、MS-271 和西玛菌素是通过在线 antiSMASH 工具发现与参考菌株具有 100%相似性的有效治疗药物。
