Clinical Pathology Department, Faculty of Veterinary Medicine, Zagazig University, 1 Al-Zeraa Street, 44511, Zagazig, Sharkia, Egypt.
Clinical Pathology Department, Faculty of Veterinary Medicine, Zagazig University, 1 Al-Zeraa Street, 44511, Zagazig, Sharkia, Egypt.
Fish Shellfish Immunol. 2021 Jan;108:147-156. doi: 10.1016/j.fsi.2020.11.028. Epub 2020 Dec 8.
The present work was designed to assess the potential hemato-biochemical protective action, immunemodulatory and antioxidant conclusions of varied concentration of white mulberry Morus alba leaves (MAL) extract supplementation on Nile tilapia (Oreochromis .niloticus). A total two hundred and forty of O. niloticus were haphazardly sorted into four groups. The control (CT) group was fed on basal diet. A group MAL, MAL and MAL was fed on 1, 3 and 5 g/kg MAL respectively for thirty days. On day thirty one, half of replicates in each group were challenged by 0.5 ml × 10Aeromonas hydrophila where, the residual replicates were kept without challenge. A. hydrophila challenged tilapias revealed anemia that alleviated by supplementation with 5 g/kg MAL also, recovers the shift of leucogram prompted by the challenge. Elevation of alkaline phosphatase, aminotransferases, lactate dehydrogenase and malondialdehyde (ALP, ALT, AST, LDH and MDA) in CT, MAL and MAL in the challenged replicates respectively where within normal at MAL. Supplementation with MAL showed more potent antioxidant and immune reaction than MAL and MAL. There were a rapid increase of immunoglobulin M, lysozymes, nitric oxide, catalase and superoxide dismutase and their allied genes expression (IgM, CAT and SOD) in MAL groups with contrast in CT challenged groups. Where in challenged groups, there was suppression in genes expression of interleukins (8 and 1 beta) and interferon ɤ (IL8. IL-1β and INFɤ). Tilapias challenged by A. hydrophila unveiled plentiful surge in the percentage of mortality in CT challenged fish (80%), followed by the groups supplemented with MAL and MAL were (73.33%) where MAL was 20%. The mortalities have been halted from the 6, 13, 14 and 15 days in, MAL, MAL, MAL, and CT correspondingly. These previous results could be fulfilled that using of MAL 5 g/kg protect tilapias from hemato-biochemical alterations and enhance its immune feedback, antioxidant defense and resistance against A. hydrophila.
本研究旨在评估不同浓度的白桑（Morus alba）叶提取物补充剂对尼罗罗非鱼（Oreochromis niloticus）的潜在血液生化保护作用、免疫调节和抗氧化作用。将 240 条罗非鱼随机分为 4 组。对照组（CT）投喂基础饲料。MAL 组、MAL 组和 MAL 组分别投喂 1、3 和 5 g/kg MAL，持续 30 天。在第 31 天，每组的一半重复被 0.5 ml×10 哈维氏弧菌（Aeromonas hydrophila）挑战，其余重复则不进行挑战。A. hydrophila 挑战的罗非鱼出现贫血，5 g/kg MAL 补充可缓解贫血，也可恢复因挑战而导致的白细胞谱变化。CT、MAL 和 MAL 组中碱性磷酸酶（ALP）、转氨酶（ALT）、乳酸脱氢酶（LDH）和丙二醛（MDA）升高，而 MAL 组正常。与 MAL 和 MAL 相比，MAL 补充剂显示出更强的抗氧化和免疫反应。MAL 组的免疫球蛋白 M（IgM）、溶菌酶、一氧化氮、过氧化氢酶和超氧化物歧化酶及其相关基因表达（IgM、CAT 和 SOD）迅速增加，而 CT 组则相反。在 CT 组中，白细胞介素（8 和 1β）和干扰素γ（IL8、IL-1β 和 INFγ）的基因表达受到抑制。被 A. hydrophila 挑战的罗非鱼死亡率在 CT 组（80%）显著增加，其次是 MAL 和 MAL 组（分别为 73.33%和 73.33%），而 MAL 组为 20%。从第 6、13、14 和 15 天起，MAL、MAL、MAL 和 CT 组的死亡率分别停止。这些结果表明，使用 5 g/kg MAL 可保护罗非鱼免受血液生化变化的影响，增强其免疫反应、抗氧化防御能力和对 A. hydrophila 的抵抗力。
