Allahverdiyeva Shabnam, Yardım Yavuz, Şentürk Zühre
Van Yuzuncu Yil University, Faculty of Science, Department of Biochemistry, 65080, Van, Turkey.
Van Yuzuncu Yil University, Faculty of Pharmacy, Department of Analytical Chemistry, 65080, Van, Turkey.
Talanta. 2021 Feb 1;223(Pt 1):121695. doi: 10.1016/j.talanta.2020.121695. Epub 2020 Sep 28.
In this paper, for the first time, the study of voltammetric determination of tetracycline antibiotic demeclocycline was conducted. The oxidation of compound was investigated using a commercially available boron-doped diamond electrode pretreated electrochemically (anodic and subsequent cathodic). Addition of anionic surfactant, sodium dodecylsulfate (SDS) and cationic surfactant, cetyltrimethylammonium bromide (CTAB) to the demeclocycline-containing electrolyte solution at pH 2.0 and 9.0, respectively, was found to improve the sensitivity of the stripping voltammetric measurements. Employing square-wave stripping mode (after 30 s accumulation at open-circuit condition) in Britton-Robinson buffer, the limits of detection were found to be 1.17 μg mL (2.3 × 10 M) for 4 × 10 SDS-containing buffer solution at pH 2, and 0.24 μg mL (4.8 × 10 M) for 1 × 10 CTAB-containing buffer solution at pH 9.0. The feasibility of the developed approach for the quantification of demeclocycline was tested in urine samples.
本文首次开展了伏安法测定四环素类抗生素地美环素的研究。使用经电化学预处理（阳极处理及随后的阴极处理）的市售掺硼金刚石电极研究了该化合物的氧化情况。分别在pH值为2.0和9.0的含地美环素的电解质溶液中添加阴离子表面活性剂十二烷基硫酸钠（SDS）和阳离子表面活性剂十六烷基三甲基溴化铵（CTAB），发现可提高溶出伏安测量的灵敏度。在Britton-Robinson缓冲液中采用方波溶出模式（在开路条件下累积30 s后），发现在pH值为2的含4×10 SDS的缓冲溶液中，检测限为1.17 μg mL（2.3×10 M），在pH值为9.0的含1×10 CTAB的缓冲溶液中，检测限为0.24 μg mL（4.8×10 M）。在尿液样本中测试了所开发的地美环素定量方法的可行性。
