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蛋白质凝聚物作为老化的麦克斯韦流体。

Protein condensates as aging Maxwell fluids.

机构信息

Max Planck Institute for the Physics of Complex Systems, Nöthnitzer Str. 38, 01187 Dresden, Germany.

Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany.

出版信息

Science. 2020 Dec 11;370(6522):1317-1323. doi: 10.1126/science.aaw4951.

Abstract

Protein condensates are complex fluids that can change their material properties with time. However, an appropriate rheological description of these fluids remains missing. We characterize the time-dependent material properties of in vitro protein condensates using laser tweezer-based active and microbead-based passive rheology. For different proteins, the condensates behave at all ages as viscoelastic Maxwell fluids. Their viscosity strongly increases with age while their elastic modulus varies weakly. No significant differences in structure were seen by electron microscopy at early and late ages. We conclude that protein condensates can be soft glassy materials that we call Maxwell glasses with age-dependent material properties. We discuss possible advantages of glassy behavior for modulation of cellular biochemistry.

摘要

蛋白质凝聚物是复杂的流体,其物质特性随时间而变化。然而,这些流体的适当流变学描述仍然缺失。我们使用基于激光镊子的主动和基于微珠的被动流变学来描述体外蛋白质凝聚物的时变材料特性。对于不同的蛋白质,凝聚物在所有年龄段都表现为粘弹性 Maxwell 流体。它们的粘度随年龄的增长而强烈增加,而弹性模量则变化微弱。在早期和晚期,电子显微镜都没有观察到结构上的显著差异。我们得出结论,蛋白质凝聚物可以是软玻璃状物质,我们称之为具有年龄依赖性材料特性的 Maxwell 玻璃。我们讨论了玻璃态行为对细胞生物化学调节的可能优势。

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