Department of Bioengineering, Cyprus International University, Northern Cyprus Via Mersin 10, Haspolat, Nicosia, Turkey.
Biotechnology Research Centre, Cyprus International University, Northern Cyprus Via Mersin 10, Haspolat, Nicosia, Turkey.
Sci Rep. 2023 May 3;13(1):7193. doi: 10.1038/s41598-023-34281-x.
Plants have been one the most valuable sources of biologically active compounds. This study investigates the chemical composition, as well as the antioxidant, antimicrobial, and cytotoxic activities of methanolic and ethanolic extracts from Juniperus sabina and Ferula communis leaves, grown in Cyprus. Total phenolic and flavonoids content of methanol and ethanol extracts were quantified. Chemical constituents of the leaf extracts were analysed using gas chromatography/mass spectrometry (GC/MS). Mome inositol was the predominant component in the J. Sabina's extracts. The most dominant component in F. communis ethanolic extract was phytol, while in FCL methanolic extract 1,3,4,5 tetrahydroxycyclohexanecarboxylic acid. Antioxidant activities were evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging ability. Antioxidant activity results revealed concentration dependent activity for methanolic and ethanolic extracts from the plant leaves. Antibacterial activity of plant extracts was tested against Gram-negative and Gram-positive bacteria using disk diffusion and minimal inhibitory concentration methods. Cytotoxic activity of plant extracts were evaluated on MCF-7 and MDA-MB-231 breast cancer cell lines, where they demonstrated their potential on the viability of both cell lines. The biological activity revealed by plants is due to the bioactive compounds found in the extracts. These bioactive components could be used as anticancer drug candidates.
植物一直是具有生物活性化合物的最有价值的来源之一。本研究调查了生长在塞浦路斯的 Juniperus sabina 和 Ferula communis 叶的甲醇和乙醇提取物的化学成分，以及抗氧化、抗菌和细胞毒性活性。甲醇和乙醇提取物中的总酚类和类黄酮含量进行了定量。使用气相色谱/质谱联用仪（GC/MS）分析了叶提取物的化学成分。在 J. Sabina 的提取物中，肌醇是主要成分。在 F. communis 乙醇提取物中，植醇是最主要的成分，而在 FCL 甲醇提取物中，1,3,4,5 四羟基环己烷羧酸是最主要的成分。通过 1,1-二苯基-2-苦基肼（DPPH）自由基清除能力评估抗氧化活性。抗氧化活性结果表明，植物叶的甲醇和乙醇提取物具有浓度依赖性活性。采用圆盘扩散法和最小抑菌浓度法测试了植物提取物对革兰氏阴性菌和革兰氏阳性菌的抗菌活性。评估了植物提取物对 MCF-7 和 MDA-MB-231 乳腺癌细胞系的细胞毒性活性，它们在这两种细胞系的活力上都表现出了潜力。植物表现出的生物活性归因于提取物中发现的生物活性化合物。这些生物活性成分可被用作抗癌药物的候选物。
