Oloomi Mana, Javadi Maryam, Asadi Karam Mohammad Reza, Khezerloo Jamil Kheirvari, Haghri Zohreh, Bouzari Saeid
Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran.
Biotechnol Rep (Amst). 2020 Nov 26;28:e00564. doi: 10.1016/j.btre.2020.e00564. eCollection 2020 Dec.
Urinary tract infections (UTIs) are induced by exogenous organisms including extraintestinal pathogenic such as (ExPEC), and , which are closely related. These organisms can colonize in the urinary tract and cause UTIs. In this study, a cross-reactive multi-epitope vaccine was designed by two constructs to stimulate the immune system (CD8+ and CD4 + T cells) against ExPEC, and strains. Uropathogenic (UPEC), and are the main bacterial cause of UTI. They were used for designing experimental candidate vaccine, and their immunogenicity and protectivity were assessed. In this study, conserved antigens from their bacterial genomes were considered, and informatics-based immunological vaccine with cross-protective T and B-cells epitopes was designed and evaluated. The vaccine candidate was used as a broad immune system inducer, and its cross-protective immunity and protectivity were confirmed in experiments.
尿路感染（UTIs）由包括肠道外致病性大肠杆菌（ExPEC）等外源生物体引起，这些生物体密切相关。这些生物体可在尿路中定植并导致尿路感染。在本研究中，通过两种构建体设计了一种交叉反应性多表位疫苗，以刺激免疫系统（CD8 +和CD4 + T细胞）对抗ExPEC、大肠杆菌和肺炎克雷伯菌菌株。尿路致病性大肠杆菌（UPEC）、大肠杆菌和肺炎克雷伯菌是尿路感染的主要细菌病因。它们被用于设计实验性候选疫苗，并评估其免疫原性和保护力。在本研究中，考虑了来自它们细菌基因组的保守抗原，并设计和评估了具有交叉保护性T和B细胞表位的基于信息学的免疫疫苗。候选疫苗用作广泛的免疫系统诱导剂，其交叉保护性免疫和保护力在实验中得到证实。
