Discipline of Genetics, School of Life Sciences, University of KwaZulu-Natal, Westville Campus, P/Bag X54001, Durban 4000, South Africa.
Department of Chemical Engineering, Mangosuthu University of Technology, Umlazi, Durban 4031, South Africa.
Viruses. 2022 Jun 30;14(7):1456. doi: 10.3390/v14071456.
Chicken anemia virus (CAV) causes severe clinical and sub-clinical infection in poultry globally and thus leads to economic losses. The drawbacks of the commercially available vaccines against CAV disease signal the need for a novel, safe, and effective vaccine design. In this study, a multiepitope vaccine (MEV) consisting of T-cell and B-cell epitopes from CAV viral proteins (VP1 and VP2) was computationally constructed with the help of linkers and adjuvant. The 3D model of the MEV construct was refined and validated by different online bioinformatics tools. Molecular docking showed stable interaction of the MEV construct with TLR3, and this was confirmed by Molecular Dynamics Simulation. Codon optimization and in silico cloning of the vaccine in pET-28a (+) vector also showed its potential expression in the K12 system. The immune simulation also indicated the ability of this vaccine to induce an effective immune response against this virus. Although the vaccine in this study was computationally constructed and still requires further in vivo study to confirm its effectiveness, this study marks a very important step towards designing a potential vaccine against CAV disease.
鸡贫血病毒（CAV）在全球范围内引起家禽的严重临床和亚临床感染，从而导致经济损失。针对 CAV 病的商业可用疫苗存在缺陷，这表明需要设计一种新型、安全和有效的疫苗。在这项研究中，借助接头和佐剂，计算构建了由 CAV 病毒蛋白（VP1 和 VP2）的 T 细胞和 B 细胞表位组成的多表位疫苗（MEV）。使用不同的在线生物信息学工具对 MEV 构建体的 3D 模型进行了细化和验证。分子对接显示 MEV 构建体与 TLR3 之间存在稳定的相互作用，这通过分子动力学模拟得到了证实。疫苗在 pET-28a（+）载体中的密码子优化和计算机克隆也表明其在 K12 系统中有潜在的表达能力。免疫模拟也表明该疫苗有能力诱导针对该病毒的有效免疫反应。尽管这项研究中的疫苗是通过计算构建的，仍需要进一步的体内研究来确认其有效性，但这项研究标志着设计针对 CAV 病的潜在疫苗迈出了非常重要的一步。
