Hauser Belinda R, Aure Marit H, Kelly Michael C, Hoffman Matthew P, Chibly Alejandro M
Matrix and Morphogenesis Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA.
Genomics and Computational Biology Core, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA.
iScience. 2020 Nov 20;23(12):101838. doi: 10.1016/j.isci.2020.101838. eCollection 2020 Dec 18.
Understanding the dynamic transcriptional landscape throughout organ development will provide a template for regenerative therapies. Here, we generated a single-cell RNA sequencing atlas of murine submandibular glands identifying transcriptional profiles that revealed cellular heterogeneity during landmark developmental events: end bud formation, branching morphogenesis, cytodifferentiation, maturation, and homeostasis. Trajectory inference analysis suggests plasticity among acinar and duct populations. We identify transcription factors correlated with acinar differentiation including , and , and loss of , and . Furthermore, we characterize two intercalated duct populations defined by either and , or . This atlas can be used to investigate specific cell functions and comparative studies predicting common mechanisms involved in development of branching organs.
了解整个器官发育过程中的动态转录图谱将为再生疗法提供一个模板。在这里，我们生成了小鼠下颌下腺的单细胞RNA测序图谱，确定了转录谱，揭示了标志性发育事件（终末芽形成、分支形态发生、细胞分化、成熟和稳态）期间的细胞异质性。轨迹推断分析表明腺泡和导管群体之间具有可塑性。我们确定了与腺泡分化相关的转录因子，包括 、 和 ，以及 、 和 的缺失。此外，我们表征了由 、 或 定义的两种闰管群体。该图谱可用于研究特定细胞功能以及预测分支器官发育中涉及的共同机制的比较研究。
