母体血清硝酪氨酸、8-异前列腺素和总抗氧化能力水平在孕前或妊娠期糖尿病。

Maternal serum nitrotyrosine, 8-isoprostane and total antioxidant capacity levels in pre-gestational or gestational diabetes mellitus.

机构信息

Department of Obstetrics, Division of Maternal-Fetal Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductive Medicine, St. Petersburg, Russia.

Faculty of Medicine, Department of Obstetrics, Gynecology and Reproduction, St. Petersburg State University, St. Petersburg, Russia.

出版信息

Gynecol Endocrinol. 2020;36(sup1):36-42. doi: 10.1080/09513590.2020.1816727.

DOI:10.1080/09513590.2020.1816727

PMID:33305672

Abstract

OBJECTIVE

To evaluate serum concentration of 8-isoprostane, nitrotyrosine (NT), and total antioxidant capacity (TAC) in pregnant women with diabetes mellitus (DM) considering preconception planning and method of diabetes correction in 11-14 and 30-34 weeks.

MATERIALS AND METHODS

The study included 130 women: T1DM (n = 40), T2DM (n = 35), gestational diabetes (GDM, n = 40) and the control group (n = 15). The serum concentrations of NT, 8-isoprostane, and TAC were measured by ELISA methods.

RESULTS

Elevated 8-isoprostane levels were observed in all patients with DM, but this biomarker's maximum values have been seen in T1DM and T2DM on insulin groups. A similar tendency was observed for the concentration of NT in both the 1st and 3rd trimesters. TAC levels showed a statistically relevant decrease in all DM groups compared to the control. The correlation analysis showed a direct correlation between HbA1c and serum 8-isoprostane levels in the 1st (r = .27) and 3rd (r = .3) pregnancy trimesters as well as inverse correlation with TAC level (r = -.48). Direct (NT, 8-isoprostane) and inverse correlations (TAC) were fixated for this biomarker concentration and preeclampsia rates.

CONCLUSION

DM in pregnancy is related to oxidative stress activation, which might lead to the development of adverse perinatal outcomes.

摘要

目的

评估患有糖尿病（DM）的孕妇在考虑受孕前计划和 11-14 周及 30-34 周时糖尿病矫正方法的情况下，血清 8-异前列腺素、硝基酪氨酸（NT）和总抗氧化能力（TAC）的浓度。

材料与方法

该研究纳入了 130 名女性：1 型糖尿病（T1DM，n=40）、2 型糖尿病（T2DM，n=35）、妊娠期糖尿病（GDM，n=40）和对照组（n=15）。采用 ELISA 法测定 NT、8-异前列腺素和 TAC 的血清浓度。

结果

所有 DM 患者均出现 8-异前列腺素水平升高，但在胰岛素组的 T1DM 和 T2DM 中观察到该生物标志物的最高值。在第一和第三孕期，NT 的浓度也呈现出类似的趋势。与对照组相比，所有 DM 组的 TAC 水平均显著降低。相关性分析显示，在第一（r=.27）和第三（r=.3）孕期，HbA1c 与血清 8-异前列腺素水平呈直接相关，与 TAC 水平呈负相关（r= -.48）。直接（NT、8-异前列腺素）和负相关（TAC）被固定为该生物标志物浓度和子痫前期发生率。