猪圆环病毒3型（PCV3）衣壳蛋白前部的高重复精氨酸是其在大肠杆菌中表达的严重障碍。
High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli.
作者信息
Liu Bing Yan, Gao Bin, Liu Meng Zhi, Zhang Ting Ting, Liu Bao Shan, Chen Ze Liang
机构信息
Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, No. 120, Dongling Road, Shenyang, 110866, Liaoning, People's Republic of China.
出版信息
AMB Express. 2020 Dec 11;10(1):214. doi: 10.1186/s13568-020-01163-8.
Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitive arginine (ΔCAP) and their codon-optimized counterparts in E. coli. These results showed that lots of repeated arginine could severely lower the expression of PCV3 capsid protein in E. coli. At the same time, the recombined truncated PCV3 capsid protein forms typic virions. The efficient expression of capsid protein is expected to serve the development of PCV3 vaccines and other studies of PCV3 capsid protein.
猪圆环病毒3型（PCV3）是在出现类似增生性坏死性皮炎（PDNS）临床症状和繁殖障碍的母猪中发现的一种新型圆环病毒。PCV3的衣壳蛋白（CAP）有望成为一种有效的疫苗候选物。在此，我们在大肠杆菌中表达了原始衣壳蛋白、无前部高度重复精氨酸的截短衣壳蛋白（ΔCAP）及其密码子优化的对应物。这些结果表明，大量重复的精氨酸会严重降低PCV3衣壳蛋白在大肠杆菌中的表达。同时，重组截短的PCV3衣壳蛋白形成典型的病毒粒子。衣壳蛋白的高效表达有望为PCV3疫苗的研发及PCV3衣壳蛋白的其他研究提供帮助。
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