Ogorodnik Evgeny, Karsai Arpad, Wang Kang-Hsin, Liu Fu-Tong, Lo Su Hao, Pinkerton Kent E, Gilbert Benjamin, Haudenschild Dominik R, Liu Gang-Yu
Biophysics Graduate Group, University of California, Davis, California 95616, United States.
Department of Chemistry, University of California, Davis, California 95616, United States.
J Phys Chem B. 2020 Dec 24;124(51):11584-11592. doi: 10.1021/acs.jpcb.0c08132. Epub 2020 Dec 11.
The interaction of long nanowires and living cells is directly related to nanowires' nanotoxicity and health impacts. Interactions of silver nanowires (AgNWs) and macrophage cell lines (NR8383) were investigated using laser scanning confocal microscopy and single cell compression (SCC). With high-resolution imaging and mechanics measurement of individual cells, AgNW-induced frustrated phagocytosis was clearly captured in conjunction with structural and property changes of cells. While frustrated phagocytosis is known for long microwires and long carbon nanotubes, this work reports first direct observations of frustrated phagocytosis of AgNWs among living cells in situ. In the case of partial penetration of AgNWs into NR8383 cells, confocal imaging revealed actin participation at the entry sites, whose behavior differs from microwire-induced frustrated phagocytosis. The impacts of frustrated phagocytosis on the cellular membrane and cytoskeleton were also quantified by measuring the mechanical properties using SCC. Taken collectively, this study reveals the structural and property characteristics of nanowire-induced frustrated phagocytosis, which deepens our understanding of nanowire-cell interactions and nanocytotoxicity.
长纳米线与活细胞的相互作用直接关系到纳米线的纳米毒性和对健康的影响。使用激光扫描共聚焦显微镜和单细胞压缩（SCC）技术研究了银纳米线（AgNWs）与巨噬细胞系（NR8383）的相互作用。通过对单个细胞的高分辨率成像和力学测量，结合细胞的结构和性质变化，清晰地捕捉到了AgNW诱导的吞噬受挫现象。虽然吞噬受挫现象在长微丝和长碳纳米管中已有报道，但这项工作首次直接观察到了活细胞中原位发生的AgNW吞噬受挫现象。在AgNW部分穿透NR8383细胞的情况下，共聚焦成像显示肌动蛋白在进入位点参与其中，其行为与微丝诱导的吞噬受挫现象不同。还通过使用SCC测量力学性能来量化吞噬受挫对细胞膜和细胞骨架的影响。综合来看，这项研究揭示了纳米线诱导的吞噬受挫现象的结构和性质特征，加深了我们对纳米线 - 细胞相互作用和纳米细胞毒性的理解。
