Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, China.
College of Animal Science and Technology, Guangxi University, Nanning, China.
Foodborne Pathog Dis. 2021 Apr;18(4):253-259. doi: 10.1089/fpd.2020.2844. Epub 2020 Dec 11.
is a protozoan with worldwide distribution that infects birds and mammals, including humans. The consumption of free-range chicken meat is a common practice in many parts of the world. However, little information is available concerning the molecular prevalence and genotypes of infection in free-range chickens intended for human consumption in China. In this study, a total of 1360 serum samples were collected from food markets in Hunan province of China for detecting antibodies by indirect hemagglutination assay. In addition, 650 brain tissues were also collected to investigate DNA by amplification of B1 gene with a seminested polymerase chain reaction (PCR), and the positive DNA samples were typed at 10 genetic markers using multilocus PCR-restriction fragment length polymorphism. Antibodies to were detected in 457 of the examined serum samples (33.6%; 95% confidence interval [CI]: 31.1-36.1), and 72 DNA samples (11.1%; 95% CI: 8.6-13.4) were positive for the B1 gene. In this study, region and age of free-range chickens were shown to be risk factors for infection ( < 0.01). Two genotypes (ToxoDB#9 and ToxoDB#52) were identified from two samples with complete genotyping results. Our study revealed a high prevalence of infection in free-range chickens intended for human consumption in Hunan province, suggesting that recommendations to consumers should be made, especially in some regions of China where consumption of undercooked chicken meat is common. This is the first genetic characterization of in free-range chickens intended for human consumption in Hunan province, China, and also the first report of genotype ToxoDB#52 in China.
是一种分布于世界各地的原生动物，感染鸟类和哺乳动物，包括人类。在世界许多地区，食用散养鸡肉是一种常见做法。然而，关于中国供人类食用的散养鸡中 感染的分子流行率和基因型，信息很少。在这项研究中，共从中国湖南省的食品市场采集了 1360 份血清样本，通过间接血凝试验检测 抗体。此外，还采集了 650 份脑组织，通过半巢式聚合酶链反应（PCR）扩增 B1 基因来检测 DNA，并使用多位点 PCR-限制性片段长度多态性对阳性 DNA 样本进行 10 个遗传标记的分型。在检测的 1360 份血清样本中，有 457 份（33.6%；95%置信区间[CI]：31.1-36.1）检测到 抗体，72 份（11.1%；95%CI：8.6-13.4）DNA 样本对 B1 基因呈阳性。在本研究中，散养鸡的区域和年龄被证明是 感染的危险因素（<0.01）。从两个具有完整基因分型结果的样本中鉴定出两种基因型（ToxoDB#9 和 ToxoDB#52）。本研究揭示了湖南省供人类食用的散养鸡中 感染的高流行率，这表明应该向消费者提出建议，特别是在中国一些普遍食用未煮熟鸡肉的地区。这是中国湖南省供人类食用的散养鸡中 感染的首次基因特征描述，也是中国首次报道基因型 ToxoDB#52。
