Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu, Kitakyushu 808-0196, Japan; Biomass Energy Technology Research Centre, Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture and Rural Affairs), Biogas Institute of Ministry of Agriculture and Rural Affairs, Section 4-13, Renmin South Road, Chengdu 610041, PR China.
Biomass Energy Technology Research Centre, Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture and Rural Affairs), Biogas Institute of Ministry of Agriculture and Rural Affairs, Section 4-13, Renmin South Road, Chengdu 610041, PR China.
Bioresour Technol. 2021 Feb;321:124497. doi: 10.1016/j.biortech.2020.124497. Epub 2020 Dec 3.
Low ability of waste sewage sludge to degrade cellulose is observed due to its less cellulolytic bacteria content. The enrichment of sewage sludge in the absence or presence of carboxymethylcellulose (CMC) was conducted to improve anaerobic digestion (AD) of cellulose in this study. Compared to initial sewage sludge (IS), enriched sludge without CMC addition (ES) displayed 69.81% higher CH yield and about 1.7-fold greater anaerobic biodegradation of cellulose. In particular, bacterial and archaeal diversities in samples inoculated with ES were significantly altered, with Ruminiclostridium and Methanobacterium as the predominant genera. Enriched sludge with CMC addition (ESC) displayed enhanced methane production at initial cellulose fermentation but showed no distinct difference compared with the control after incubation 24 days. These findings suggest that enrichment of waste sewage sludge without CMC addition is more beneficial for promoting AD of cellulose, providing a novel insight for efficient energy utilization of lignocellulosic wastes.
由于废污泥中纤维素分解菌含量较少，其降解纤维素的能力较低。本研究通过在不存在或存在羧甲基纤维素（CMC）的情况下对污水污泥进行富集，以提高纤维素的厌氧消化（AD）。与初始污泥（IS）相比，未添加 CMC 的富集污泥（ES）的 CH 产量高出 69.81%，纤维素的厌氧生物降解率提高了约 1.7 倍。特别是，用 ES 接种的样品中的细菌和古菌多样性发生了显著变化，其中瘤胃菌属和甲烷杆菌属是主要属。添加 CMC 的富集污泥（ESC）在初始纤维素发酵时表现出增强的甲烷生成，但在孵育 24 天后与对照相比没有明显差异。这些发现表明，不添加 CMC 的废污泥富集更有利于促进纤维素的 AD，为木质纤维素废物的有效能源利用提供了新的见解。
