ICAR-National Bureau of Agriculturally Important Microorganisms (NBAIM), Maunath Bhanjan, Uttar Pradesh 275103, India.
School of Life and Environmental Sciences, University of Sydney, NSW 2006, Australia.
Bioresour Technol. 2021 Feb;321:124495. doi: 10.1016/j.biortech.2020.124495. Epub 2020 Dec 3.
Eukaryotic microalgae are a rich source of commercially important metabolites including lipids, pigments, sugars, amino acids and enzymes. However, their inherent genetic potential is usually not enough to support high level production of metabolites of interest. In order to move on from the traditional approach of improving product yields by modification of the cultivation conditions, understanding the metabolic pathways leading to the synthesis of the bioproducts of interest is crucial. Identification of new targets for strain engineering has been greatly facilitated by the rapid development of high-throughput sequencing and spectroscopic techniques discussed in this review. Despite the availability of high throughput analytical tools, examples of gathering and application of proteomic and metabolomic data for metabolic engineering of microalgae are few and mainly limited to lipid production. The present review highlights the application of contemporary proteomic and metabolomic techniques in eukaryotic microalgae for redesigning pathways for enhanced production of algal metabolites.
真核微藻是具有商业价值的代谢产物的丰富来源，包括脂类、色素、糖、氨基酸和酶。然而，它们固有的遗传潜力通常不足以支持目标代谢产物的高水平生产。为了摆脱通过改变培养条件来提高产品产量的传统方法，了解导致目标生物制品合成的代谢途径至关重要。本综述中讨论的高通量测序和光谱技术的快速发展极大地促进了新目标菌株工程的鉴定。尽管有高通量分析工具，但用于微藻代谢工程的蛋白质组学和代谢组学数据的收集和应用的例子很少，并且主要限于脂质生产。本综述重点介绍了当代蛋白质组学和代谢组学技术在真核微藻中的应用，用于重新设计途径以增强藻类代谢产物的生产。
