Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA; DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Bioresour Technol. 2022 Feb;345:126529. doi: 10.1016/j.biortech.2021.126529. Epub 2021 Dec 9.
Microalgae are promising sustainable resources because of their ability to convert CO into biofuels and chemicals directly. However, the industrial production and economic feasibility of microalgal bioproducts are still limited. As such, metabolic engineering approaches have been undertaken to enhance the productivities of microalgal bioproducts. In the last decade, impressive advances in microalgae metabolic engineering have been made by developing genetic engineering tools and multi-omics analysis. This review presents comprehensive microalgal metabolic pathways and metabolic engineering strategies for producing lipids, long chain-polyunsaturated fatty acids, terpenoids, and carotenoids. Additionally, promising metabolic engineering approaches specific to target products are summarized. Finally, this review discusses current challenges and provides future perspectives for the effective production of chemicals and fuels via microalgal metabolic engineering.
微藻因其将 CO2 直接转化为生物燃料和化学品的能力而成为有前途的可持续资源。然而，微藻生物制品的工业生产和经济可行性仍然有限。因此，已经采取了代谢工程方法来提高微藻生物制品的生产力。在过去的十年中，通过开发遗传工程工具和多组学分析，在微藻代谢工程方面取得了令人瞩目的进展。本综述介绍了用于生产脂质、长链多不饱和脂肪酸、萜类化合物和类胡萝卜素的全面的微藻代谢途径和代谢工程策略。此外，还总结了针对特定目标产物的有前途的代谢工程方法。最后，本文讨论了当前的挑战，并为通过微藻代谢工程有效生产化学品和燃料提供了未来的展望。
