BMP2 对于颞下颌关节的骨软骨组织的产后维持是必需的。
BMP2 Is Required for Postnatal Maintenance of Osteochondral Tissues of the Temporomandibular Joint.
机构信息
Division of Orthodontics, University of Connecticut Health Center, Farmington, CT, USA.
出版信息
Cartilage. 2021 Dec;13(2_suppl):734S-743S. doi: 10.1177/1947603520980158. Epub 2020 Dec 14.
OBJECTIVE
Bone morphogenetic protein 2 (BMP2) plays important roles in cartilage growth and development. Paradoxically, elevated levels of BMP2 leads to hypertrophic differentiation and osteoarthritis of cartilage. We examined the loss of BMP2 in cells expressing aggrecan of the mandibular condyle and knee.
DESIGN
Three-week-old --positive mice and their Cre-negative littermates were treated with tamoxifen and raised until 3 or 6 months. We also investigated the direct effects of BMP2 on chondrocytes . Cells from the mandibular condyle of mice were treated with recombinant human BMP2 (rhBMP2) or rhNoggin (inhibitor of BMP2 signaling).
RESULTS
Conditional deletion of BMP2 caused breakage of the cartilage integrity in the mandibular condyle of mice from both age groups, accompanied by a decrease in cartilage thickness, matrix synthesis, mineralization, chondrocyte proliferation, and increased expression of degeneration markers, while the effects at articular cartilage were not significant. results revealed that rhBMP2 increased chondrocyte proliferation, mineralization, and differentiation, while noggin induced opposite effects.
CONCLUSIONS
In conclusion, BMP2 is essential for postnatal maintenance of the osteochondral tissues of the mandibular condyle.
目的
骨形态发生蛋白 2（BMP2）在软骨生长和发育中发挥重要作用。矛盾的是，BMP2 水平升高会导致软骨肥大分化和骨关节炎。我们研究了下颌髁和膝关节中表达聚集蛋白聚糖的细胞中 BMP2 的缺失。
设计
3 周龄的 Cre 阳性小鼠及其 Cre 阴性同窝仔鼠用他莫昔芬处理，并饲养至 3 或 6 个月。我们还研究了 BMP2 对软骨细胞的直接作用。从小鼠下颌髁中分离的细胞用重组人 BMP2（rhBMP2）或 rhNoggin（BMP2 信号抑制剂）处理。
结果
BMP2 的条件性缺失导致来自两个年龄组的小鼠下颌髁软骨完整性的破坏，伴随着软骨厚度、基质合成、矿化、软骨细胞增殖减少和退变标志物表达增加，而对关节软骨的影响不显著。结果表明，rhBMP2 增加了软骨细胞的增殖、矿化和分化，而 noggin 则诱导了相反的效果。
结论
综上所述，BMP2 对于下颌髁的成骨软骨组织的出生后维持是必需的。
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