College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Int J Biol Macromol. 2021 Jan 31;168:215-222. doi: 10.1016/j.ijbiomac.2020.12.050. Epub 2020 Dec 9.
A novel nanoparticle (NP) delivery carrier for curcumin based on electrostatic 6-deoxy-6-arginine modified chitosan (DAC) assembled by γ-poly-glutamic acid (γ-PGA) was prepared. The NP structure was evaluated using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Interactions between DAC and γ-PGA were characterized using Fourier transform infrared spectroscopy (FT-IR). The sustained release kinetics of curcumin-loaded NPs was investigated in simulated gastrointestinal fluids. After exposed to heating, pH, and NaCl aqueous solution, the stabilities of both normal and curcumin-loaded NPs were determined. The results showed that NPs achieved a high encapsulation efficiency (79.5%) and loading capacity (11.31%) for curcumin. The curcumin-loaded NPs displayed a sustained release profile under simulated gastrointestinal conditions. Under certain pH (3-9), salt (0-100 mM), and temperature (30 - 60 °C) conditions, the vehicles of curcumin showed better stability. This demonstrates that NPs can be used as stable carriers for curcumin.
一种新型基于静电的纳米粒子（NP）递送载体，用于将姜黄素基于γ-聚谷氨酸（γ-PGA）组装的 6-脱氧-6-精氨酸修饰壳聚糖（DAC）。使用扫描电子显微镜（SEM）和透射电子显微镜（TEM）评估 NP 结构。使用傅立叶变换红外光谱（FT-IR）表征 DAC 和 γ-PGA 之间的相互作用。在模拟胃肠道液中研究了载姜黄素 NP 的缓释动力学。在暴露于加热、pH 和 NaCl 水溶液后，确定了正常和载姜黄素 NP 的稳定性。结果表明，NP 对姜黄素的包封效率（79.5%）和载药量（11.31%）很高。在模拟胃肠道条件下，载姜黄素 NP 显示出持续释放的特征。在一定的 pH（3-9）、盐（0-100 mM）和温度（30-60°C）条件下，姜黄素的载体表现出更好的稳定性。这表明 NP 可以用作姜黄素的稳定载体。
