精氨酸改性壳聚糖/γ-聚谷氨酸纳米粒的制备、表征及其作为姜黄素载体的应用。
Formation, characterization and application of arginine-modified chitosan/γ-poly glutamic acid nanoparticles as carrier for curcumin.
机构信息
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
出版信息
Int J Biol Macromol. 2021 Jan 31;168:215-222. doi: 10.1016/j.ijbiomac.2020.12.050. Epub 2020 Dec 9.
A novel nanoparticle (NP) delivery carrier for curcumin based on electrostatic 6-deoxy-6-arginine modified chitosan (DAC) assembled by γ-poly-glutamic acid (γ-PGA) was prepared. The NP structure was evaluated using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Interactions between DAC and γ-PGA were characterized using Fourier transform infrared spectroscopy (FT-IR). The sustained release kinetics of curcumin-loaded NPs was investigated in simulated gastrointestinal fluids. After exposed to heating, pH, and NaCl aqueous solution, the stabilities of both normal and curcumin-loaded NPs were determined. The results showed that NPs achieved a high encapsulation efficiency (79.5%) and loading capacity (11.31%) for curcumin. The curcumin-loaded NPs displayed a sustained release profile under simulated gastrointestinal conditions. Under certain pH (3-9), salt (0-100 mM), and temperature (30 - 60 °C) conditions, the vehicles of curcumin showed better stability. This demonstrates that NPs can be used as stable carriers for curcumin.
一种新型基于静电的纳米粒子（NP）递送载体，用于将姜黄素基于γ-聚谷氨酸（γ-PGA）组装的 6-脱氧-6-精氨酸修饰壳聚糖（DAC）。使用扫描电子显微镜（SEM）和透射电子显微镜（TEM）评估 NP 结构。使用傅立叶变换红外光谱（FT-IR）表征 DAC 和 γ-PGA 之间的相互作用。在模拟胃肠道液中研究了载姜黄素 NP 的缓释动力学。在暴露于加热、pH 和 NaCl 水溶液后，确定了正常和载姜黄素 NP 的稳定性。结果表明，NP 对姜黄素的包封效率（79.5%）和载药量（11.31%）很高。在模拟胃肠道条件下，载姜黄素 NP 显示出持续释放的特征。在一定的 pH（3-9）、盐（0-100 mM）和温度（30-60°C）条件下，姜黄素的载体表现出更好的稳定性。这表明 NP 可以用作姜黄素的稳定载体。
Zotero 插件