Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.
Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.
Physiol Behav. 2021 Mar 1;230:113285. doi: 10.1016/j.physbeh.2020.113285. Epub 2020 Dec 10.
Cesarean section (CS) has been associated with an increased risk of mental disorders in the offspring. This could possibly be explained by an inadequate microbial colonization early in life with a consequential disturbed gut-brain interaction. To investigate the link between delivery mode and behavior and develop a suitable animal model for further research of the gut-brain axis, the aim of this study was to characterize the gut microbiota (GM) together with the behavioral response in various behavioral tests in CS-delivered mice. We hypothesized that mice delivered by CS would present with disturbances in normal physiological behavior possibly due to an inadequate microbial colonization. C57BL/6 mice delivered by CS or vaginal delivery (VD) were cross fostered and, as adults, observed for anxiety-related behavior in the open field test, social deficits in a sociability test and compulsive behavior in the marble burying test. GM was analyzed by 16S rRNA gene amplicon sequencing. The open field test showed that CS-delivered mice had a decreased activity and accelerated defecation compared to VD-delivered mice. In addition, CS-delivered female mice spend less time interacting with cage mates in the sociability test, whereas there was no effect of CS delivery on the average number of marbles buried. In conclusion, CS-delivered mice had a more pronounced anxiety-like behavior and showed less preference for sociability in female offspring.
剖宫产（CS）与后代精神障碍的风险增加有关。这可能是由于生命早期微生物定植不足，导致肠道-大脑相互作用紊乱。为了研究分娩方式与行为之间的联系，并为进一步研究肠道-大脑轴开发合适的动物模型，本研究旨在描述 CS 分娩小鼠的肠道微生物群（GM）及其在各种行为测试中的行为反应。我们假设 CS 分娩的小鼠可能会因微生物定植不足而出现正常生理行为紊乱。CS 分娩或阴道分娩（VD）的 C57BL/6 小鼠被交叉寄养，并在成年后观察其在旷场试验中的焦虑相关行为、社交能力试验中的社交缺陷和大理石掩埋试验中的强迫行为。通过 16S rRNA 基因扩增子测序分析 GM。旷场试验显示，与 VD 分娩的小鼠相比，CS 分娩的小鼠活动减少，排便加速。此外，CS 分娩的雌性小鼠在社交能力试验中与笼内同伴的互动时间减少，而 CS 分娩对平均大理石掩埋数量没有影响。总之，CS 分娩的小鼠表现出更明显的焦虑样行为，并且雌性后代的社交偏好较低。
