Maschek Sina, Østergaard Theresa Helena, Krych Lukasz, Zachariassen Line Fisker, Sørensen Dorte Bratbo, Junker Mentzel Caroline M, Hansen Axel Kornerup, Sjögren Jan Magnus, Barfod Kenneth Klingenberg
Food Science, University of Copenhagen, Frederiksberg C, Denmark.
Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Ridebanevej 9, Frederiksberg C, Denmark.
J Eat Disord. 2025 May 13;13(1):82. doi: 10.1186/s40337-025-01276-0.
Anorexia nervosa (AN) is a complex and serious mental disorder, which may affect individuals of all ages and sex, but primarily affecting young women. The disease is characterized by a disturbed body image, restrictive eating behavior, and a lack of acknowledgment of low body weight. The underlying causes of AN remain largely unknown, and current treatment options are limited to psychotherapy and nutritional support. This paper investigates the impact of Fecal Microbiota Transplants (FMT) from patients with AN on food intake, body weight, behavior, and gut microbiota into antibiotic-treated mice. Two rounds of FMT were performed using AN and control (CO) donors. During the second round of FMT, a subset of mice received gut microbiota (GM) from a different donor type. This split-group cross-over design was chosen to demonstrate any recovery effect of FMT from a non-eating disorder state donor. The first FMT, from donors with AN, resulted in lower food intake in mice without affecting body weight. Analysis of GM showed significant differences between AN and CO mice after FMT1, before cross-over. Specific bacterial genera and families Ruminococcaceae, Lachnospiraceae, and Faecalibacterium showed different abundances in AN and CO receiving mice. Behavioral tests showed decreased locomotor activity in AN mice after FMT1. After FMT2, serum analysis revealed higher levels of appetite-influencing hormones (PYY and leptin) in mice receiving AN-GM. Overall, the results suggest that AN-GM may contribute to altered food intake and appetite regulation, which can be ameliorated with FMT from a non-eating disorder state donor potentially offering FMT as a supportive treatment for AN.
神经性厌食症（AN）是一种复杂且严重的精神障碍，可能影响所有年龄和性别的个体，但主要影响年轻女性。该疾病的特征是身体形象紊乱、限制性饮食行为以及对低体重缺乏认知。AN的潜在病因在很大程度上仍然未知，目前的治疗选择仅限于心理治疗和营养支持。本文研究了来自AN患者的粪便微生物群移植（FMT）对经抗生素治疗的小鼠的食物摄入量、体重、行为和肠道微生物群的影响。使用AN供体和对照（CO）供体进行了两轮FMT。在第二轮FMT期间，一部分小鼠接受了来自不同供体类型的肠道微生物群（GM）。选择这种分组交叉设计是为了证明来自非饮食失调状态供体的FMT的任何恢复效果。第一次FMT来自患有AN的供体，导致小鼠食物摄入量降低，但不影响体重。GM分析显示，在交叉前的FMT1后，AN小鼠和CO小鼠之间存在显著差异。特定的细菌属和科，如瘤胃球菌科、毛螺菌科和粪杆菌属，在接受AN和CO的小鼠中表现出不同的丰度。行为测试显示，FMT1后AN小鼠的运动活动减少。FMT2后，血清分析显示接受AN-GM的小鼠中影响食欲的激素（PYY和瘦素）水平较高。总体而言，结果表明AN-GM可能导致食物摄入量和食欲调节的改变，而来自非饮食失调状态供体的FMT可能会改善这种情况，这可能为AN提供FMT作为一种支持性治疗方法。
