A Ratiometric Fluorescent Probe for NH Having a Large Detection Range Based upon Coumarin with Multiple Applications.

Although hydrazine (NH) is a versatile chemical used in many applications, it is toxic, and its leakage may pose a threat to both human health and environments. Consequently, the monitoring of NH is significant. This study reports a one-step synthesis for coumarin-based ratiometric fluorescent probe (FP) , with acetyl as the recognition group. Selected deprotection of the acetyl group via NH released the coumarin fluorophore, which recovered the intramolecular charge transfer process, which caused a prominent fluorescent, ratiometric response. demonstrated the advantages of high selectivity, a strong capacity for anti-interference, a low limit of detection (LOD) (0.16 μM), a large linear detection range (0-500 μM), and a wide effective pH interval (6-12) in NH detection. Furthermore, the probe enabled quantitative NH verifications in environmental water specimens in addition to qualitative detection of NH in various soils and of gaseous NH. Finally, the probe ratiometrically monitored NH in living cells having low cytotoxicity.