Cheng Xianghan, Wang Xuefei, Zhang Ang, Wang Panpan, Chen Qianyi, Ma Tingting, Li Wanping, Liang Yanying, Sun Xiangyu, Fang Yulin
College of Enology, College of Food Science and Engineering, Technology Center of State Forestry and Grassland Administration, Shaanxi Engineering Research Center for Viti-Viniculture, Heyang Viti-Viniculture Station, Northwest A&F University, Yangling 712100, China.
Technology Centre of Qinhuangdao Customs, Qinhuangdao, Hebei 066004, China.
J Agric Food Chem. 2020 Dec 30;68(52):15390-15402. doi: 10.1021/acs.jafc.0c05565. Epub 2020 Dec 15.
The effects of foliar phenylalanine application during veraison (FPV) on phenolic biosynthesis and correlation between phenolic compositions and antioxidant activities in Cabernet Sauvignon grown in field and greenhouse were investigated. Solutions with 69 and 138 mg N/vine phenylalanine (Pe1 and Pe2, respectively) and an aqueous solution without nitrogen (CK) were sprayed three times during veraison. FPV significantly improved antioxidant activities in grapes using the two culture methods. The most contributory phenolic compositions to antioxidant activities were anthocyanins and stilbenes following FPV compared with CK. Phenylalanine metabolism, abscisic acid content, and expression levels of , , , , and in the phenolic synthesis pathway were increased from the first FPV to harvest. Although Pe2 significantly increased total phenolic contents than Pe1, antioxidant parameters were not markedly affected by the phenylalanine dose. Our finding revealed that FPV was a useful fertilization method to enhance antioxidant activities in grapes in nitrogen-deficient vineyards.
研究了在转色期叶面喷施苯丙氨酸（FPV）对田间和温室种植的赤霞珠葡萄酚类物质生物合成的影响，以及酚类成分与抗氧化活性之间的相关性。在转色期，分别用含69和138毫克氮/株的苯丙氨酸溶液（分别为Pe1和Pe2）和无氮水溶液（CK）进行三次喷雾处理。FPV显著提高了两种栽培方式下葡萄的抗氧化活性。与CK相比，转色期叶面喷施苯丙氨酸后，对抗氧化活性贡献最大的酚类成分是花青素和芪类化合物。从第一次喷施苯丙氨酸到收获，苯丙氨酸代谢、脱落酸含量以及酚类合成途径中相关基因的表达水平均有所增加。虽然Pe2比Pe1显著增加了总酚含量，但抗氧化参数并未受到苯丙氨酸剂量的显著影响。我们的研究结果表明，在缺氮葡萄园，转色期叶面喷施苯丙氨酸是提高葡萄抗氧化活性的一种有效施肥方法。
