Magaz Adrián, Spencer Ben F, Hardy John G, Li Xu, Gough Julie E, Blaker Jonny J
Department of Materials and Henry Royce Institute, The University of Manchester, Manchester M13 9PL, United Kingdom.
Institute of Materials Research and Engineering (IMRE), Agency for Science Technology and Research (A*STAR), Singapore 138634 Singapore.
ACS Biomater Sci Eng. 2020 Dec 14;6(12):6906-6916. doi: 10.1021/acsbiomaterials.0c01239. Epub 2020 Nov 16.
Peripheral nerve injury is a common consequence of trauma with low regenerative potential. Electroconductive scaffolds can provide appropriate cell growth microenvironments and synergistic cell guidance cues for nerve tissue engineering. In the present study, electrically conductive scaffolds were prepared by conjugating poly (3,4-ethylenedioxythiophene)-polystyrene sulfonate (PEDOT-PSS) or dimethyl sulfoxide (DMSO)-treated PEDOT-PSS on electrospun silk scaffolds. Conductance could be tuned by the coating concentration and was further boosted by DMSO treatment. Analogue NG108-15 neuronal cells were cultured on the scaffolds to evaluate neuronal cell growth, proliferation, and differentiation. Cellular viability was maintained on all scaffold groups while showing comparatively better metabolic activity and proliferation than neat silk. DMSO-treated PEDOT-PSS functionalized scaffolds partially outperformed their PEDOT-PSS counterparts. Differentiation assessments suggested that these PEDOT-PSS assembled silk scaffolds could support neurite sprouting, indicating that they show promise to be used as a future platform to restore electrochemical coupling at the site of injury and preserve normal nerve function.
周围神经损伤是创伤常见的后果，其再生潜力较低。导电支架可为神经组织工程提供合适的细胞生长微环境和协同的细胞引导线索。在本研究中，通过将聚（3,4 - 乙撑二氧噻吩）-聚苯乙烯磺酸盐（PEDOT - PSS）或二甲基亚砜（DMSO）处理的PEDOT - PSS共轭到静电纺丝丝支架上来制备导电支架。电导率可通过涂层浓度进行调节，并通过DMSO处理进一步提高。在支架上培养类似NG108 - 15的神经元细胞，以评估神经元细胞的生长、增殖和分化。所有支架组均维持了细胞活力，同时与纯丝相比显示出相对更好的代谢活性和增殖能力。经DMSO处理的PEDOT - PSS功能化支架在一定程度上优于其PEDOT - PSS对应物。分化评估表明，这些PEDOT - PSS组装的丝支架能够支持神经突萌发，这表明它们有望作为未来的平台，用于恢复损伤部位的电化学耦合并维持正常神经功能。
