Martiniano Sabrina Evelin, Fernandes Letícia Alves, Alba Edith Mier, Philippini Rafael Rodrigues, Tabuchi Stephanie Caroline Tavares, Kieliszek Marek, Santos Júlio César Dos, da Silva Silvio Silvério
Engineering School of Lorena, University of São Paulo, Lorena 12.602-810, SP, Brazil.
Department of Food Biotechnology and Microbiology, Institute of Food Sciences, Warsaw University of Life Sciences-SGGW, 02-776 Warsaw, Poland.
Metabolites. 2020 Dec 13;10(12):508. doi: 10.3390/metabo10120508.
The production of biomolecules using agro-industrial by-products as feedstock is a growing trend worldwide. Selenium (Se) is a trace element essential for health, and the Se-enrichment of yeast biomass can enhance its benefits. This study investigated the feasibility of the production of Se-enriched biomass using a medium composed of corn bran and soybean bran acid hydrolysates as carbon and nitrogen sources in a stirred-tank reactor. After hydrolysis, hydrolysates presented complex composition and high concentrations of sugars, proteins, and minerals. The use of a stirred-tank bioreactor leads to the production of 9 g/L biomass enriched with 236.93 μg/g Se, and 99% cell viability. Likewise, the combination of sugarcane molasses and soybean bran hydrolysate was effective for cell growth of a probiotic strain of with a 24.08% β-glucan content. The results demonstrated that starchy acid hydrolysates are low-cost and efficient substrates for the production of yeast biomass and derivate products and may contribute to further studies for a sustainable development of biorefinery technologies.
利用农业工业副产品作为原料生产生物分子在全球范围内是一种日益增长的趋势。硒（Se）是一种对健康至关重要的微量元素，酵母生物质的富硒可以增强其益处。本研究调查了在搅拌釜反应器中使用由玉米麸皮和大豆麸皮酸水解物作为碳源和氮源组成的培养基生产富硒生物质的可行性。水解后，水解物呈现出复杂的成分以及高浓度的糖、蛋白质和矿物质。使用搅拌釜生物反应器可生产出9 g/L富含236.93 μg/g硒的生物质，且细胞活力达99%。同样，甘蔗 molasses和大豆麸皮水解物的组合对β-葡聚糖含量为24.08%的益生菌菌株的细胞生长有效。结果表明，淀粉酸水解物是生产酵母生物质及其衍生产品的低成本且高效的底物，可能有助于生物炼制技术可持续发展的进一步研究。
