College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China.
Int J Mol Sci. 2020 Dec 13;21(24):9487. doi: 10.3390/ijms21249487.
The KT/HAK/KUP (HAK) family is the largest potassium (K) transporter family in plants, which plays key roles in K uptake and homeostasis, stress resistance, and root and embryo development. However, the HAK family has not yet been characterized in . In this study, 40 putative HAK genes () are identified and divided into four groups (Groups I-III and V) on the basis of phylogenetic analysis. Gene structure analysis revealed 10 conserved intron insertion sites across different groups. Collinearity analysis demonstrated that both allopolyploidization and small-scale duplication events contributed to the large expansion of . Transcription factor (TF)-binding network construction, -element analysis, and microRNA prediction revealed that the expression of is regulated by multiple factors. Analysis of RNA-sequencing data further revealed extensive expression profiles of the in groups II, III, and V, with limited expression in group I. Compared with group I, most of the in groups II, III, and V were more upregulated by hormone induction based on RNA-sequencing data. Reverse transcription-quantitative polymerase reaction analysis revealed that the expression of eight of groups I and V was markedly upregulated under K-deficiency treatment. Collectively, our results provide valuable information and key candidate genes for further functional studies of .
KT/HAK/KUP（HAK）家族是植物中最大的钾（K）转运蛋白家族，在 K 吸收和稳态、抗逆性以及根和胚胎发育中发挥关键作用。然而，在 中尚未对 HAK 家族进行特征描述。在这项研究中，基于系统发育分析，鉴定出了 40 个推定的 HAK 基因（），并将其分为四个组（I 组-IV 组和 V 组）。基因结构分析显示，不同组之间存在 10 个保守的内含子插入位点。共线性分析表明，异源多倍化和小规模重复事件都导致了 的大量扩张。转录因子（TF）结合网络构建、顺式作用元件分析和 microRNA 预测表明，的表达受到多种因素的调控。对 RNA-seq 数据的分析进一步揭示了 在 II 组、III 组和 V 组中的广泛表达谱，而在 I 组中的表达有限。与 I 组相比，II 组、III 组和 V 组中的大多数 在基于 RNA-seq 数据的激素诱导下表达上调。反转录定量聚合酶链反应分析显示，在低钾处理下，I 组和 V 组的八个 的表达明显上调。总之，我们的研究结果为进一步研究 的功能提供了有价值的信息和关键候选基因。
