Schliwa M, Weber K, Porter K R
J Cell Biol. 1981 May;89(2):267-75. doi: 10.1083/jcb.89.2.267.
Melanophores of the angelfish, Pterophyllum scalare, were studied in an attempt to demonstrate the existence of actin in these cells although microfilaments had previously not been found. By use of a variety of procedures, including immunofluorescence microscopy of intact and detergent-extracted cells, transmission electron microscopy, high voltage electron microscopy of whole-mount preparations, and labeling with heavy meromyosin-subfragment 1, the presence of a loose cortical mesh of actin filaments is demonstrated. In addition, a more parallel array of filaments is detected in microspike- and microvillus-like surface projections. There seem to be no changes in the arrangement of these filaments as a function of the state of pigment distribution. No actin filaments could be found in association with pigment granules or microtubules in more central cell portions. For reasons presently unknown, the preservation of the cortical filament network in lysed cell preparations depends strongly on the presence of an intact microtubular system. The involvement of this subplasmalemmal actin filament network in pigment granule transport remains unclear.
为了证明肌动蛋白在神仙鱼（Pterophyllum scalare）细胞中的存在，尽管此前未发现微丝，但对其黑素细胞进行了研究。通过使用多种方法，包括对完整细胞和经去污剂处理的细胞进行免疫荧光显微镜检查、透射电子显微镜检查、整装标本的高压电子显微镜检查以及用重酶解肌球蛋白亚片段1进行标记，证明了肌动蛋白丝存在松散的皮质网状结构。此外，在微刺状和微绒毛状表面突起中检测到更平行排列的丝。这些丝的排列似乎不会随色素分布状态而变化。在细胞更中心的部分，未发现与色素颗粒或微管相关的肌动蛋白丝。由于目前尚不清楚的原因，裂解细胞制剂中皮质丝网络的保存强烈依赖于完整微管系统的存在。质膜下肌动蛋白丝网络在色素颗粒运输中的作用仍不清楚。
