Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh.
Department of Pharmacy, Forman Christian College (A Chartered University), Lahore, Pakistan.
Biosci Rep. 2021 Jan 29;41(1). doi: 10.1042/BSR20203022.
Merremia vitifolia (Burm.f.) Hallier f., an ethnomedicinally important plant, used in the tribal areas to treat various ailments including fever, headache, eye inflammation, rheumatism, dysentery, jaundice and urinary diseases. The present study explored the biological efficacy of the aqueous fraction of M. vitifolia leaves (AFMV) through in vitro and in vivo experimental models. The thrombolytic and anti-arthritic effects of AFMV were evaluated by using the clot lysis technique and inhibition of protein denaturation technique, respectively. The anti-nociceptive activity of AFMV was investigated in Swiss Albino mice by acetic acid-induced writhing test and formalin-induced paw licking test. The antioxidant activities of AFMV, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and total reducing power, were also tested. The qualitative phytochemical assays exhibited AFMV contains secondary metabolites such as alkaloid, carbohydrate, flavonoid, tannin, triterpenoids and phenols. In addition, AFMV showed strong antioxidant effects with the highest scavenging activity (IC50 146.61 µg/mL) and reducing power was increased with a dose-dependent manner. AFMV also revealed notable clot lysis effect and substantial anti-arthritic activity at higher doses (500 µg/mL) as compared with the control. The results demonstrated a promising reduction of the number of writhing and duration of paw licking in acetic acid-induced writhing test and formalin-induced paw licking test in a dose-dependent manner, respectively. In conclusion, AFMV provides the scientific basis of its folkloric usage, suggesting it as the vital source of dietary supplement.
马蹄金（Burm.f.）Hallier f.，一种具有民族医学重要性的植物，在部落地区被用于治疗各种疾病，包括发热、头痛、眼炎、风湿、痢疾、黄疸和尿路疾病。本研究通过体外和体内实验模型探索了马蹄金叶水提物（AFMV）的生物学功效。分别采用血栓溶解技术和抑制蛋白质变性技术评价 AFMV 的溶栓和抗关节炎作用。通过醋酸诱导扭体试验和甲醛诱导舔爪试验研究 AFMV 的抗伤害作用。还测试了 AFMV 的抗氧化活性，包括 2,2-二苯基-1-苦基肼（DPPH）自由基和总还原能力。定性植物化学分析表明，AFMV 含有生物碱、碳水化合物、类黄酮、单宁、三萜和酚类等次生代谢物。此外，AFMV 表现出较强的抗氧化作用，最高清除活性（IC50 为 146.61 µg/mL）和还原能力呈剂量依赖性增加。与对照相比，AFMV 还显示出显著的血栓溶解作用和较高剂量（500 µg/mL）的显著抗关节炎活性。结果表明，在醋酸诱导扭体试验和甲醛诱导舔爪试验中，扭体次数和舔爪时间均呈剂量依赖性减少。综上所述，AFMV 为其民间用途提供了科学依据，表明它是膳食补充剂的重要来源。
