Kocadal Onur, Pepe Murad, Akyurek Nalan, Gunes Zafer, Surer Hatice, Aksahin Ertugrul, Ogut Betul, Aktekin Cem Nuri
Department of Orthopedics and Traumatology, Yeditepe University Faculty of Medicine, Istanbul, Turkey.
Department of Orthopedics and Traumatology, Alanya Research and Training Hospital, Alanya Alaaddin Keykubat Unversity, Alanya, Turkey.
Clin Shoulder Elb. 2019 Jun 1;22(2):79-86. doi: 10.5397/cise.2019.22.2.79. eCollection 2019 Jun.
Increased oxidative stress and inflammation play a critical role in the etiopathogenesis of chronic tendinopathy. Melatonin is an endogenous molecule that exhibits antioxidant and anti-inflammatory activity. The aim of this study was to evaluate the biochemical and histopathological effects of exogenous melatonin administrations in supraspinatus overuse tendinopathy.
Fifty rats were divided into the following four groups: cage activity, melatonin treatment, corticosteriod therapy, and control. Melatonin (10 mg/kg, intraperitoneal; twice a day) and triamcinolone (0.3 mg/kg, subacromial; weekly) were administered to the treatment groups after the overuse period. Biochemical and histopathological evaluations were performed on serum samples and biopsies obtained from rats. Plasma inducible nitric oxide synthase (iNOS), vascular endothelial growth factor (VEGF), total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) levels were evaluated biochemically.
The TAS, TOS, OSI, iNOS, and VEGF values were significantly lower than the pre-treatment levels in rats receiving exogenous melatonin treatment (3 or 6 weeks) (<0.05). TOS, iNOS, VEGF, and OSI values after 3 weeks of triamcinolone administration, and TOS, VEGF, and OSI levels after 6 weeks of triamcinolone application, were significantly lower than the pre-treatment levels (<0.05).
Exogenous melatonin application in overuse tendinopathy reduces oxidative stress and inflammation. Melatonin might be an alternative potential molecule to corticosteroids in the treatment of chronic tendinopathy.
氧化应激和炎症增加在慢性肌腱病的发病机制中起关键作用。褪黑素是一种具有抗氧化和抗炎活性的内源性分子。本研究的目的是评估外源性褪黑素给药对冈上肌过度使用性肌腱病的生化和组织病理学影响。
50只大鼠分为以下四组：笼内活动组、褪黑素治疗组、皮质类固醇治疗组和对照组。在过度使用期后，对治疗组给予褪黑素（10mg/kg，腹腔注射；每日两次）和曲安奈德（0.3mg/kg，肩峰下注射；每周一次）。对从大鼠获得的血清样本和活检组织进行生化和组织病理学评估。生化评估血浆诱导型一氧化氮合酶（iNOS）、血管内皮生长因子（VEGF）、总抗氧化状态（TAS）、总氧化状态（TOS）和氧化应激指数（OSI）水平。
接受外源性褪黑素治疗（3或6周）的大鼠的TAS、TOS、OSI、iNOS和VEGF值显著低于治疗前水平（<0.05）。曲安奈德给药3周后的TOS、iNOS、VEGF和OSI值，以及曲安奈德应用6周后的TOS、VEGF和OSI水平显著低于治疗前水平（<0.05）。
在过度使用性肌腱病中应用外源性褪黑素可降低氧化应激和炎症。在慢性肌腱病的治疗中，褪黑素可能是皮质类固醇的一种潜在替代分子。
