Chair of Microbiology, Jagiellonian University, Medical College, Kraków, Poland.
Chair of Medical Biochemistry, Jagiellonian University, Medical College, Kraków, Poland.
Acta Biochim Pol. 2020 Dec 17;67(4):623-628. doi: 10.18388/abp.2020_5413.
Several species of Ureaplasma bacteria are known to be present in the urogenital tract of humans, in both healthy individuals and symptomatic patients. These pathogens are associated with urogenital tract infections, infertility problems and spontaneous abortion in humans. The present study involved 77 strains of Ureaplasma species (Ureaplasma spp.), including 21 Ureaplasma urealyticum (U. urealyticum) strains and 56 Ureaplasma parvum (U. parvum) strains. Lipoic acid (LA) and its reduced form dihydrolipoic acid (DHLA) are synthesized in all prokaryotic and eukaryotic cells. Research of recent years increasingly points to therapeutic properties of exogenously supplemented LA. In our study, we examined for the first time the effect of LA on the bacteria multiplication and its bactericidal activity against U. urealyticum and U. parvum. The LA concentrations used were: 1200 µg/ml, 120 µg/ml, and 12 µg/ml. The titer for each strain of Ureaplasma spp. was estimated using the color changing units (CCU) assay. For CCU measurements, a series of 10-fold dilutions of each cell culture in 0.9% NaCl (titration) was prepared and 1 CCU/ml was defined as the highest dilution of cells at which color change was detected. The strongest bacteriostatic and bactericidal effect of LA was observed at a concentration of 1200 µg/ml. In contrast, at lower LA concentrations, stimulation of the bacteria multiplication was noted for 14% of the total number of strains tested. Taken together, the current data provide novel findings about potential beneficial antimicrobial effects of LA.
已知有几种脲原体细菌存在于人类的泌尿生殖道中，包括健康个体和有症状的患者。这些病原体与人类泌尿生殖道感染、不孕问题和自然流产有关。本研究涉及 77 株脲原体（Ureaplasma spp.），包括 21 株解脲脲原体（U. urealyticum）和 56 株微小脲原体（U. parvum）。硫辛酸（LA）及其还原形式二氢硫辛酸（DHLA）在所有原核和真核细胞中合成。近年来的研究越来越多地指出外源性补充 LA 的治疗特性。在我们的研究中，我们首次研究了 LA 对细菌增殖及其对解脲脲原体和微小脲原体的杀菌活性的影响。使用的 LA 浓度为：1200µg/ml、120µg/ml 和 12µg/ml。使用变色单位（CCU）测定法估计每种脲原体菌株的滴度。对于 CCU 测量，制备了每种细胞培养物在 0.9%NaCl 中的 10 倍稀释系列（滴定），并将 1CCU/ml 定义为检测到颜色变化的最高细胞稀释度。LA 的最强抑菌和杀菌作用在 1200µg/ml 的浓度下观察到。相比之下，在较低的 LA 浓度下，测试的总菌株数中有 14%观察到细菌增殖的刺激。总的来说，目前的数据提供了关于 LA 潜在有益的抗菌作用的新发现。
