School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur, India.
Department of Computer Science and Engineering, Indian Institute of Technology Kharagpur, Kharagpur, India.
J Biophotonics. 2021 Apr;14(4):e202000357. doi: 10.1002/jbio.202000357. Epub 2020 Dec 29.
Imaging the structural modifications of underlying tissues is vital to monitor wound healing. Optical coherence tomography (OCT) images high-resolution sub-surface information, but suffers a loss of intensity with depth, limiting quantification. Hence correcting the attenuation loss is important. We performed swept source-OCT of full-thickness excision wounds for 300 days in mice skin. We used single-scatter attenuation models to determine and correct the attenuation loss in the images. The phantom studies established the correspondence of corrected-OCT intensity (reflectivity) with matrix density and hydration. We histologically validated the corrected-OCT and measured the wound healing rate. We noted two distinct phases of healing-rapid and steady-state. We also detected two compartments in normal scars using corrected OCT that otherwise were not visible in the OCT scans. The OCT reflectivity in the scar compartments corresponded to distinct cell populations, mechanical properties and composition. OCT reflectivity has potential applications in evaluating the therapeutic efficacy of healing and characterizing scars.
对潜在组织的结构变化进行成像对于监测伤口愈合至关重要。光学相干断层扫描（OCT）图像可以提供高分辨率的亚表面信息，但随着深度的增加，强度会降低，限制了定量分析。因此，校正衰减损失很重要。我们对小鼠皮肤的全层切除伤口进行了扫频源 OCT 检测，持续 300 天。我们使用单散射衰减模型来确定和校正图像中的衰减损失。在体研究建立了校正 OCT 强度（反射率）与基质密度和水合作用的对应关系。我们对校正 OCT 进行了组织学验证，并测量了伤口愈合率。我们注意到愈合有两个不同的阶段——快速和稳态。我们还使用校正 OCT 检测到正常瘢痕中的两个隔室，而在 OCT 扫描中则无法看到这些隔室。瘢痕隔室中的 OCT 反射率与不同的细胞群、机械特性和组成相对应。OCT 反射率在评估愈合的治疗效果和表征瘢痕方面具有潜在的应用价值。
