Kim Jaeyoung, Kim Suhyun, Choi Woo June
Research Institute for Skin Image, Korea University College of Medicine, Seoul 08308, Republic of Korea.
Department of Dermatology and Skin Science, University of British Columbia, Vancouver, BC V6T 1Z1, Canada.
Bioengineering (Basel). 2023 Apr 27;10(5):538. doi: 10.3390/bioengineering10050538.
A diabetic wound presents a severe risk of infections and other complications because of its slow healing. Evaluating the pathophysiology during wound healing is imperative for wound care, necessitating a proper diabetic wound model and assay for monitoring. The adult zebrafish is a rapid and robust model for studying human cutaneous wound healing because of its fecundity and high similarities to human wound repair. OCTA as an assay can provide three-dimensional (3D) imaging of the tissue structure and vasculature in the epidermis, enabling monitoring of the pathophysiologic alterations in the zebrafish skin wound. We present a longitudinal study for assessing the cutaneous wound healing of the diabetic adult zebrafish model using OCTA, which is of importance for the diabetes research using the alternative animal models. We used non-diabetic ( = 9) and type 1 diabetes mellitus (DM) adult zebrafish models ( = 9). The full-thickness wound was generated on the fish skin, and the wound healing was monitored with OCTA for 15 days. The OCTA results demonstrated significant differences between diabetic and non-diabetic wound healing, involving delayed tissue remodeling and impaired angiogenesis for the diabetic wound, leading to slow wound recovery. The adult zebrafish model and OCTA technique may benefit long-term metabolic disease studies using zebrafish for drug development.
糖尿病伤口由于愈合缓慢，存在严重的感染和其他并发症风险。评估伤口愈合过程中的病理生理学对于伤口护理至关重要，这需要一个合适的糖尿病伤口模型和监测检测方法。成年斑马鱼因其繁殖力强且与人类伤口修复高度相似，是研究人类皮肤伤口愈合的快速且强大的模型。光学相干断层扫描血管造影术（OCTA）作为一种检测方法，可以提供表皮组织结构和脉管系统的三维（3D）成像，从而能够监测斑马鱼皮肤伤口的病理生理变化。我们开展了一项纵向研究，使用OCTA评估成年糖尿病斑马鱼模型的皮肤伤口愈合情况，这对于使用替代动物模型进行糖尿病研究具有重要意义。我们使用了非糖尿病（n = 9）和1型糖尿病（DM）成年斑马鱼模型（n = 9）。在鱼皮肤上制造全层伤口，并使用OCTA监测伤口愈合15天。OCTA结果显示糖尿病伤口和非糖尿病伤口愈合之间存在显著差异，包括糖尿病伤口的组织重塑延迟和血管生成受损，导致伤口恢复缓慢。成年斑马鱼模型和OCTA技术可能有益于利用斑马鱼进行药物开发的长期代谢疾病研究。
