Zhu Mengqi, Huang Yuting, Bian Saiyan, Song Qianqian, Zhang Jie, Zheng Wenjie, Cheng Chun
Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, 20 Xisi Road, 226001 Nantong, Jiangsu, China.
Medical school of Nantong University, 19 Qixiu Road, Nantong 226001, China.
Curr Mol Pharmacol. 2021 Oct 25;14(4):498-508. doi: 10.2174/1874467213666201217115854.
Understanding organogenesis, disorders, and repairing processes is particularly important for understanding the disease occurrence and developing treatment approaches. At present, liver- related studies are mainly conducted using in vivo models and cell lines, making it difficult to generalize the full picture of the structural characteristics and functions of human organs. Organoid is a three-dimensional (3D) culture system in vitro, which holds the promise to establish various disease models and conduct in-depth research by generating organ-like tissues in a dish. Recent advances in human liver organoids have provided a deeper understanding of this complex organ. In this review, we provide a systematic overview of the construction methods of organoids, focusing on their applications in the hepatic organogenesis and various liver disease models, as well as the limitations of current models. The development of organoid models is proving to be crucial in future liver research.
了解器官发生、疾病及修复过程对于理解疾病的发生和制定治疗方法尤为重要。目前，肝脏相关研究主要使用体内模型和细胞系进行，这使得全面概括人体器官的结构特征和功能变得困难。类器官是一种体外三维（3D）培养系统，有望通过在培养皿中生成类器官组织来建立各种疾病模型并进行深入研究。人类肝脏类器官的最新进展为深入了解这个复杂器官提供了帮助。在本综述中，我们系统概述了类器官的构建方法，重点介绍了它们在肝脏器官发生和各种肝脏疾病模型中的应用，以及当前模型的局限性。类器官模型的发展在未来肝脏研究中被证明至关重要。
