Department of General Surgery, the A#liated Hospital of Northwest University, Xi'an No. 3, Xi'an, Shaanxi China.
Department of General Surgery, the A#liated Hospital of Northwest University, Xi'an No. 3, Xi'an, Shaanxi China
Ann Clin Lab Sci. 2020 Nov;50(6):734-738.
Hepatocellular carcinoma (HCC) is a high-level invasive and metastatic malignancy with a low survival rate. Accumulating evidence has proved that circular RNAs (cirRNAs) function as potential biomarkers for diagnosis or prognosis in various cancer, including HCC. In the present study, we aimed to explore a novel biomarker, circ_0067934 for diagnosis and prognosis of HCC so as to contribute to therapeutic strategies. In the present study, the expression levels of circ_0067934 in HCC patients and paired controls were determined by real-time quantitative polymerase chain reaction (qRT-PCR) analysis. The correlation between different circ_0067934 expressions with clinicopathological factors of HCC patients was analyzed by one-way analysis of variance (ANOVA). Then, we examined the potential diagnostic value of circ_0067934 by measuring the receiver operating characteristic (ROC) curves. Finally, the overall survival rate evaluated by Spearman's rank correlation coefficient of HCC patients with different circ_0067934 was compared in order to present the prognostic value of circ_0067934 in HCC. From the results, qRT-PCR analysis revealed that circ_0067934 was significantly up-regulated in HCC tissues compared with adjacent non-tumor tissues. Moreover, circ_0067934 positively correlated with tumor size (=0.0025), TNM stage (<0.0001) and microvascular invasion (<0.0001). However, there were no significant differences in gender, age, family history, diabetes, ALT, AST, AFP and AKP. Meanwhile, the ROC curves presented that circ_0067934 had favorable specificity and sensitivity with the area under the curves of 0.8412 (95% CI=0.7881 to 0.8943). Furthermore, the upregulation of circ_0067934 was associated with unfavorable prognosis in patients with HCC. In conclusion, circ_0067934 may function as a potential biomarker for the diagnosis and prognosis of HCC.
肝细胞癌 (HCC) 是一种高水平侵袭性和转移性恶性肿瘤，其生存率较低。越来越多的证据证明，环状 RNA (circRNAs) 可作为各种癌症包括 HCC 的诊断或预后的潜在生物标志物。在本研究中，我们旨在探索一种新型生物标志物 circ_0067934 用于 HCC 的诊断和预后，以有助于治疗策略。在本研究中，通过实时定量聚合酶链反应 (qRT-PCR) 分析测定 HCC 患者和配对对照中 circ_0067934 的表达水平。通过单向方差分析 (ANOVA) 分析不同 circ_0067934 表达与 HCC 患者临床病理因素的相关性。然后，通过测量受试者工作特征 (ROC) 曲线来检验 circ_0067934 的潜在诊断价值。最后，通过 Spearman 秩相关系数比较不同 circ_0067934 的 HCC 患者的总生存率，以呈现 circ_0067934 在 HCC 中的预后价值。结果表明，qRT-PCR 分析显示 circ_0067934 在 HCC 组织中明显高于相邻非肿瘤组织。此外，circ_0067934 与肿瘤大小呈正相关 (=0.0025)、TNM 分期(<0.0001)和微血管侵犯(<0.0001)。然而，性别、年龄、家族史、糖尿病、ALT、AST、AFP 和 AKP 之间没有显著差异。同时，ROC 曲线显示，circ_0067934 具有良好的特异性和敏感性，曲线下面积为 0.8412(95%CI=0.7881 至 0.8943)。此外，circ_0067934 的上调与 HCC 患者的不良预后相关。总之，circ_0067934 可能作为 HCC 诊断和预后的潜在生物标志物。
