一种用于检测鸡B细胞的酶联免疫吸附测定法。
An enzyme-linked immunosorbent assay for detecting the chicken B cells.
作者信息
Benda V, Angelisová P, Jandlová T, Mach O, Slezáková P
机构信息
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
出版信息
Folia Biol (Praha). 1986;32(5):325-33.
PMID:3333489
Abstract
Two modifications of an indirect enzyme immunoassay described here allow the discernment of chicken B and T cells when a polyclonal rabbit anti-chicken Ig antibody or a monoclonal antibody reactive with chicken IgM and IgG heavy chains is used. Comparison of both types of antibody in ELISA and in indirect immunofluorescence suggests that they can readily be exploited for detection of lymphoid cells with surface immunoglobulin markers.
摘要
本文所述间接酶免疫测定的两种改进方法，在使用多克隆兔抗鸡Ig抗体或与鸡IgM和IgG重链反应的单克隆抗体时，能够区分鸡的B细胞和T细胞。在酶联免疫吸附测定（ELISA）和间接免疫荧光中对这两种抗体进行比较表明，它们可轻易用于检测带有表面免疫球蛋白标记的淋巴细胞。
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