Xu Jian-Hua, Lo Yangming Martin, Chang Wen-Chang, Huang Da-Wei, Wu James Swi-Bea, Jhang Yu-Yuan, Huang Wen-Chung, Ko Chih-Yuan, Shen Szu-Chuan
Department of Tumor Surgery, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China.
Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China.
Evid Based Complement Alternat Med. 2020 Dec 1;2020:6644253. doi: 10.1155/2020/6644253. eCollection 2020.
L. (RTL) has been used as a folk medicine to cure diabetes in Asia. RTL was previously reported to alleviate hyperglycemia, insulin resistance (IR), abnormal hepatic detoxification, and liver steatosis. However, the potential bioactive compounds of RTL have still not been identified. The aim of this study was to investigate the bioactive compounds in RTL ethyl acetate (EA) fractions by using a glucose uptake assay in TNF--treated mouse FL83B hepatocytes to discover a mechanism by which to improve IR. The bioactive compounds were identified by the high-performance liquid chromatography (HPLC) assay. Using the Sephadex LH20 gel packing chromatography column, the EAF5 fraction was isolated from RTL and significantly increased glucose uptake in TNF--treated FL83B cells. Moreover, the MCI gel packing chromatography column separated EAF5 into five subfractions and had no significant cytotoxic effect in FL83B cells when treated at the concentration of 25 g/ml. Among the subfractions, EAF5-5 markedly enhanced glucose uptake in TNF--treated FL83B cells. The possible bioactive compounds of the EAF5-5 fraction that were identified by the HPLC assay include syringic acid, -coumaric acid, and cirsimaritin. The bioactive compound with the best effect of increasing glucose uptake was -coumaric acid, but its effect alone was not as good as the combined effect of all three compounds of the EAF5-5 fraction. Thus, we speculate that the antidiabetic effect of RTL may be the result of multiple active ingredients.
在亚洲，罗汉果（RTL）一直被用作治疗糖尿病的民间药物。此前有报道称罗汉果可缓解高血糖、胰岛素抵抗（IR）、肝脏解毒异常和肝脂肪变性。然而，罗汉果潜在的生物活性化合物尚未得到鉴定。本研究的目的是通过在肿瘤坏死因子-α（TNF-α）处理的小鼠FL83B肝细胞中进行葡萄糖摄取试验，研究罗汉果乙酸乙酯（EA）组分中的生物活性化合物，以发现改善胰岛素抵抗的机制。通过高效液相色谱（HPLC）分析鉴定生物活性化合物。使用葡聚糖LH20凝胶填充色谱柱，从罗汉果中分离出EAF5组分，该组分在TNF-α处理的FL83B细胞中显著增加了葡萄糖摄取。此外，MCI凝胶填充色谱柱将EAF5分离为五个亚组分，在25μg/ml浓度下处理时对FL83B细胞没有显著的细胞毒性作用。在这些亚组分中，EAF5-5在TNF-α处理的FL83B细胞中显著增强了葡萄糖摄取。通过HPLC分析鉴定的EAF5-5组分可能的生物活性化合物包括丁香酸、对香豆酸和 cirsimaritin。增加葡萄糖摄取效果最佳的生物活性化合物是对香豆酸，但其单独作用不如EAF5-5组分中所有三种化合物的联合作用。因此，我们推测罗汉果的抗糖尿病作用可能是多种活性成分共同作用的结果。
