Huang Da-Wei, Lo Yangming Martin, Chang Wen-Chang, Lin Chia-Yu, Chen Jou-An, Wu James Swi-Bea, Huang Wen-Chung, Shen Szu-Chuan
Department of Biotechnology and Food Technology Southern Taiwan University of Science and Technology Tainan City Taiwan.
Institute for Advanced Study Shenzhen University Shenzhen China.
Food Sci Nutr. 2020 Sep 9;8(10):5710-5716. doi: 10.1002/fsn3.1868. eCollection 2020 Oct.
a L. (RTL) exhibits phytochemical activities and has been used as a folk medicine for curing diabetes mellitus in East Asia for decades. This study investigated the effect of RTL aqueous and ethanolic extracts on nonalcoholic fatty liver disease (NAFLD) and hepatic lipid accumulation in high-fat diet (HFD) and streptozotocin (STZ)-induced type 2 diabetes mellitus (T2DM) rats. Administration of RTL aqueous extract (RTLW) or ethanolic extract (RTLE) at dosage of 100 or 400 mg/kg body weight for 4 weeks was carried out in HFD/STZ-induced T2DM rats. Liver weight, adipose (epididymal and perirenal adipose tissues) weight, hepatic triglyceride level, and de novo lipogenesis (DNL)-associated protein expression were monitored after scarification. The results revealed that RTLW and RTLE reduced relative liver weight and relative fat weights in HFD/STZ-induced T2DM rats. RTLW and RTLE also ameliorated NAFLD and hepatic triglyceride (TG) accumulation in diabetic rats. Moreover, hepatic DNL-regulated enzymes such as sterol regulatory element-binding protein-1 (SREBP1) and fatty acid synthase (FAS) expression were significantly suppressed by RTLE (100 and 400 mg/kg body weight) in diabetic rats. The evidences of this study suggest that RTL possesses potential on alleviating NAFLD and lipid accumulation via regulating DNL in the liver of HFD/STZ-induced T2DM rats.
a L.（RTL）具有植物化学活性，在东亚作为治疗糖尿病的民间药物已使用数十年。本研究调查了RTL水提取物和乙醇提取物对高脂饮食（HFD）和链脲佐菌素（STZ）诱导的2型糖尿病（T2DM）大鼠非酒精性脂肪性肝病（NAFLD）和肝脏脂质积累的影响。对HFD/STZ诱导的T2DM大鼠以100或400 mg/kg体重的剂量给予RTL水提取物（RTLW）或乙醇提取物（RTLE），持续4周。在处死后监测肝脏重量、脂肪（附睾和肾周脂肪组织）重量、肝脏甘油三酯水平和从头脂肪生成（DNL）相关蛋白表达。结果显示，RTLW和RTLE降低了HFD/STZ诱导的T2DM大鼠的相对肝脏重量和相对脂肪重量。RTLW和RTLE还改善了糖尿病大鼠的NAFLD和肝脏甘油三酯（TG）积累。此外，RTLE（100和400 mg/kg体重）显著抑制了糖尿病大鼠肝脏中DNL调节酶如固醇调节元件结合蛋白-1（SREBP1）和脂肪酸合酶（FAS）的表达。本研究证据表明，RTL在通过调节HFD/STZ诱导的T2DM大鼠肝脏中的DNL来减轻NAFLD和脂质积累方面具有潜力。
