Safitri Anna, Tirto Sari Dewi Ratih, Refsilangi Bigram, Roosdiana Anna, Fatchiyah Fatchiyah
Chemistry Department, Brawijaya University, Malang, Indonesia.
Research Centre of Natural Genetic Resources (SMONAGENES), Brawijaya University, Malang, Indonesia.
Vet Med Int. 2021 Nov 10;2021:6938433. doi: 10.1155/2021/6938433. eCollection 2021.
Diabetes mellitus (DM) is a serious worldwide health threat since the number of people with DM is forecasted to grow annually. Thus, effective and affordable treatment is urgently needed. Our previous studies used n-hexane and hydroethanolic root extracts of L. which significantly affected diabetic rats. In this study, aqueous L. root extracts were used as treatments for the diabetic rat model and their effects were evaluated. Diabetes was generated by the administration of streptozotocin (STZ) at 20 mg/kg within 5 sequential days. Male Wistar rats were orally treated with the extracts and standard drug (metformin 200 mg/kg) and vehicle every day for 4 weeks. Hypoglycemic effects were assessed for normal, diabetic control, standard, and extract-treated groups. Histopathology was also carried out for the pancreatic, hepatic, and kidney tissues. The progression of diabetes was considerably diminished after extract treatment. In treated rats, the highest dose of extract induced a decline in blood glucose and serum malondialdehyde (MDA) levels at 25% and 35%, respectively. Furthermore, aqueous extract of L. treatment decreased MDA levels in the pancreas by 12%. Histologic examination of the organ tissues of diabetic rats showed deteriorating alterations. After treatment, histopathological damages to the tissues and cells were reversed. The results of the experiments recommend that aqueous extract of L. has antidiabetic effects on STZ-induced diabetic rats; nevertheless, a higher dose of the aqueous extracts is needed to achieve more significant results.
糖尿病（DM）是一种严重的全球性健康威胁，因为预计糖尿病患者人数每年都会增长。因此，迫切需要有效且经济实惠的治疗方法。我们之前的研究使用了L.的正己烷和水乙醇根提取物，它们对糖尿病大鼠有显著影响。在本研究中，L.根的水提取物被用作糖尿病大鼠模型的治疗药物，并评估了其效果。通过连续5天以20mg/kg的剂量注射链脲佐菌素（STZ）来诱导糖尿病。雄性Wistar大鼠每天口服提取物、标准药物（二甲双胍200mg/kg）和赋形剂，持续4周。对正常组、糖尿病对照组、标准组和提取物治疗组评估降血糖效果。还对胰腺、肝脏和肾脏组织进行了组织病理学检查。提取物治疗后，糖尿病的进展明显减缓。在治疗的大鼠中，最高剂量的提取物分别使血糖和血清丙二醛（MDA）水平下降了25%和35%。此外，L.的水提取物治疗使胰腺中的MDA水平降低了12%。糖尿病大鼠器官组织的组织学检查显示有恶化的改变。治疗后，组织和细胞的组织病理学损伤得到了逆转。实验结果表明，L.的水提取物对STZ诱导的糖尿病大鼠具有抗糖尿病作用；然而，需要更高剂量的水提取物才能取得更显著的效果。
