Bartlett P N, Whitaker R G
Department of Chemistry, University of Warwick, Coventry, UK.
Biosensors. 1987;3(6):359-79. doi: 10.1016/0265-928x(87)80018-4.
There are now a number of distinct strategies which can be employed to make amperometric enzyme electrodes. These include the use of homogeneous mediators, modified electrodes and organic conducting salts. In this paper we review these strategies and discuss their application to NAD(P)H dependent dehydrogenase and flavoprotein based biosensors. In addition we discuss recent work on the immobilisation of glucose oxidase in polypyrrole, poly-N-methylpyrrole, polyaniline and polyphenol films electrochemically grown at the electrode surface and on the covalent attachment of redox mediators to glucose oxidase in order to achieve direct electron transfer to the electrode.
现在有许多不同的策略可用于制造电流型酶电极。这些策略包括使用均相介质、修饰电极和有机导电盐。在本文中，我们回顾了这些策略，并讨论了它们在基于NAD(P)H依赖脱氢酶和黄素蛋白的生物传感器中的应用。此外，我们还讨论了近期关于将葡萄糖氧化酶固定在电极表面电化学生长的聚吡咯、聚-N-甲基吡咯、聚苯胺和多酚薄膜中的工作，以及将氧化还原介质共价连接到葡萄糖氧化酶上以实现向电极的直接电子转移的工作。
